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Blood, 1 July 2007, Vol. 110, No. 1, pp. 441-449. Prepublished online as a Blood First Edition Paper on March 20, 2007; DOI 10.1182/blood-2006-12-065623. This Article Full Text Full Text (PDF) Supplemental Tables and Figures All Versions of this Article: blood-2006-12-065623v1 110/1/441    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Seggewiss, R. Articles by Douek, D. C. Search for Related Content PubMed PubMed Citation Articles by Seggewiss, R. Articles by Douek, D. C. Related Collections Transplantation Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  TRANSPLANTATION Keratinocyte growth factor augments immune reconstitution after autologous hematopoietic progenitor cell transplantation in rhesus macaques. Ruth Seggewiss1, Karin Loré2, F. Javier Guenaga3, Stefania Pittaluga5, Joseph Mattapallil4, Catherine K. Chow6, Richard A. Koup2, Kevin Camphausen9, Martha C. Nason7, Martin Meier-Schellersheim8, Robert E. Donahue1, Bruce R. Blazar10, Cynthia E. Dunbar1, and Daniel C. Douek3 1 Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health/Department of Health and Human Services 2 Immunology Laboratory 3 Human Immunology Section 4 ImmunoTechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases/National Institutes of Health/Department of Health and Human Services, Bethesda, MD; 5 Hematopathology Section, National Cancer Institute, National Institutes of Health/Department of Health and Human Services 6 Diagnostic Radiology, Clinical Center, National Institutes of Health/Department of Health and Human Services, Bethesda, MD; 7 Biostatistics Research Branch, National Institute of Allergy and Infectious Diseases/National Institutes of Health/Department of Health and Human Services 8 Laboratory of Immunology, National Institute of Allergy and Infectious Diseases/National Institutes of Health/Department of Health and Human Services 9 Radiation Oncology Branch, National Cancer Institute, National Institutes of Health/Department of Health and Human Services, Bethesda, MD; 10 Cancer Center and the Department of Pediatrics, Division of Blood and Marrow Transplantation, University of Minnesota Cancer Center, Minneapolis Opportunistic infections contribute to morbidity and mortality after peripheral blood progenitor cell (PBPC) transplantation and are related to a deficient T-cell compartment. Accelerated T-cell reconstitution may therefore be clinically beneficent. Keratinocyte growth factor (KGF) has been shown to protect thymic epithelial cells in mice. Here, we evaluated immune reconstitution after autologous CD34+ PBPC transplantation in rhesus macaques conditioned with myeloablative total body irradiation in the absence or presence of single pretotal body irradiation or repeated peritransplant KGF administration. All KGF-treated animals exhibited a well-preserved thymic architecture 12 months after graft. In contrast, thymic atrophy was observed in the majority of animals in the control group. The KGF-treated animals showed higher frequencies of naive T cells in lymph nodes after transplantation compared with the control animals. The animals given repeated doses of KGF showed the highest levels of T-cell receptor excision circles (TRECs) and the lowest frequencies of Ki67+ T cells, which suggest increased thymic-dependent reconstitution in these animals. Of note, the humoral response to a T-cell–dependent neo-antigen was significantly higher in the KGF-treated animals compared with the control animals. Thus, our findings suggest that KGF may be a useful adjuvant therapy to augment T-cell reconstitution after human PBPC transplantation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: E. Kwon High-TECh thymus Blood, June 15, 2008; 111(12): 5421 - 5422. [Full Text] [PDF] L. Cicin-Sain, I. Messaoudi, B. Park, N. Currier, S. Planer, M. Fischer, S. Tackitt, D. Nikolich-Zugich, A. Legasse, M. K. Axthelm, et al. Dramatic increase in naive T cell turnover is linked to loss of naive T cells from old primates PNAS, December 11, 2007; 104(50): 19960 - 19965. [Abstract] [Full Text] [PDF]

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