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Blood, 1 July 2007, Vol. 110, No. 1, pp. 74-81. Prepublished online as a Blood First Edition Paper on March 19, 2007; DOI 10.1182/blood-2006-08-034447. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2006-08-034447v1 110/1/74    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Dao, M. A. Articles by Verfaillie, C. M. Search for Related Content PubMed PubMed Citation Articles by Dao, M. A. Articles by Verfaillie, C. M. Related Collections Hematopoiesis and Stem Cells Transplantation Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Biology of umbilical cord blood progenitors in bone marrow niches Mo A. Dao1,2, Michael H. Creer3, Jan A. Nolta2, and Catherine M. Verfaillie1,4 1 Stem Cell Institute, University of Minnesota Medical School, Minneapolis; 2 Department of Internal Medicine, Division of Oncology, Hematopoietic Development and Malignancy Program, Washington University School of Medicine, St Louis, MO; 3 Departments of Pathology and Laboratory Medicine, Saint Louis University School of Medicine, St Louis, MO; 4 Stamcel Instituut Leuven, Katholieke Universiteit Leuven, Belgium Within the bone marrow (BM), hematopoietic progenitor cells (HPCs) are localized in poorly oxygenated niches where they interact with the surrounding osteoblasts (OBs) through VLA4/VCAM-1 engagement, and are exposed to interleukin-6 (IL-6), stem cell factor (SCF), and chemokines such as CXCL12 (OB factors). Umbilical cord (UC) is more highly oxygenated that the BM microenvironment. When UC-HPCs are exposed to the 2% to 3% O2 concentration found in the bone endosteum, their survival is significantly decreased. However, engagement of VLA-4 integrins on UCB-derived CD34+ cells reduced cell death in 2% to 3% O2 conditions, which was associated with an increase in phospho-Ser473 AKT and an increase in phospho-Ser9 GSK3b. Consistent with the role of GSK3b in destabilizing beta-catenin, there was more cytoplasmic beta-catenin in UC-HPCs exposed to 2% to 3% O2 on fibronectin, compared with suspension culture. UC-HPCs cultured at 2% to 3% O2 with OB factors showed an increase in nuclear beta-catenin and persistence of a small pool of CD34+38– HPCs. CFU assays followed by surface phenotyping of the plated colonies showed improved maintenance of mixed lineage colonies with both erythroid and megakaryocytic precursors. These studies provide a biologic perspective for how UC-derived HPCs adapt to the bone endosteum, which is low in oxygen and densely populated by osteoblasts. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. E. Kendall, J. Najbauer, H. F. Johnston, M. Z. Metz, S. Li, M. Bowers, E. Garcia, S. U. Kim, M. E. Barish, K. S. Aboody, et al. Neural Stem Cell Targeting of Glioma Is Dependent on Phosphoinositide 3-Kinase Signaling Stem Cells, June 1, 2008; 26(6): 1575 - 1586. [Abstract] [Full Text] [PDF] J.-P. Fermand Initial Therapy for Multiple Myeloma: Role of Stem Cell Transplantation ASCO Educational Book, January 1, 2008; 2008(1): 375 - 379. [Abstract] [Full Text] [PDF] Y.-Y. Jang and S. J. Sharkis A low level of reactive oxygen species selects for primitive hematopoietic stem cells that may reside in the low-oxygenic niche Blood, October 15, 2007; 110(8): 3056 - 3063. [Abstract] [Full Text] [PDF]

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