SEARCH:   Advanced

Blood, 15 December 2007, Vol. 110, No. 13, pp. 4161-4164. Prepublished online as a Blood First Edition Paper on September 19, 2007; DOI 10.1182/blood-2007-06-097907. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2007-06-097907v1 110/13/4161    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Saleh, S. Articles by Lewin, S. R. Search for Related Content PubMed PubMed Citation Articles by Saleh, S. Articles by Lewin, S. R. Related Collections Brief Reports Chemokines, Cytokines, and Interleukins Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  CHEMOKINES, CYTOKINES, AND INTERLEUKINS Brief Report CCR7 ligands CCL19 and CCL21 increase permissiveness of resting memory CD4+ T cells to HIV-1 infection: a novel model of HIV-1 latency Suha Saleh1, Ajantha Solomon1, Fiona Wightman1, Miranda Xhilaga1, Paul U. Cameron1,3, and Sharon R. Lewin1,3 Departments of1 Medicine and 2 Immunology, Monash University, Melbourne; and 3 Infectious Diseases Unit, Alfred Hospital, Melbourne, Australia Latent HIV-1 infection of resting memory CD4+ T cells represents the major barrier to HIV-1 eradication. To determine whether the CCR7 ligands involved in lymphocyte migration can alter HIV-1 infection of resting CD4+ T cells, we infected purified resting CD4+ T cells after incubation with the chemokines CCL19 and CCL21. Incubation with CCL19 or CCL21 did not alter markers of T-cell activation or proliferation. However, after HIV-1 infection of CCL19- or CCL21-treated CD4+ T-cells, we observed low-level HIV-1 production but high concentrations of integrated HIV-1 DNA, approaching that seen in mitogen-stimulated T-cell blasts. Restimulation of CCL19-treated infected CD4+ T cells resulted in virus production consistent with establishment of postintegration latency. CCR7 ligands facilitate efficient entry of HIV-1 into resting CD4+ T cells. These studies demonstrate a unique action of the chemokines CCL19 and CCL21 and provide a novel model with which to study HIV-1 latency in vitro. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

	Blood Online is supported in part by Genentech BioOncology and Biogen Idec
	Copyright © 2007 by American Society of Hematology         Online ISSN: 1528-0020