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Blood, 15 December 2007, Vol. 110, No. 13, pp. 4526-4534. Prepublished online as a Blood First Edition Paper on September 13, 2007; DOI 10.1182/blood-2007-01-067801. This Article Full Text Full Text (PDF) Supplemental Appendix and Figure All Versions of this Article: blood-2007-01-067801v1 110/13/4526    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Twu, Y.-C. Articles by Yu, L.-C. Search for Related Content PubMed PubMed Citation Articles by Twu, Y.-C. Articles by Yu, L.-C. Related Collections Transfusion Medicine Gene Expression Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  TRANSFUSION MEDICINE I branching formation in erythroid differentiation is regulated by transcription factor C/EBP Yuh-Ching Twu1, Chie-Pein Chen2,3, Chuang-Yi Hsieh1, Cheng-Hwai Tzeng4,5, Chien-Feng Sun6,7, Shih-Hsin Wang8, Mau-Sun Chang3, and Lung-Chih Yu1,8 1 Institute of Biochemical Sciences, National Taiwan University, Taipei; 2 Division of High Risk Pregnancy, Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei; 3 Department of Medical Research, Mackay Memorial Hospital, Taipei; 4 Section of Transfusion Medicine, Department of Medicine, Taipei Veterans General Hospital, Taipei; 5 College of Medicine, National Yang-Ming University, Taipei; 6 Department of Clinical Pathology, Linkou Medical Center, Chang Gung Memorial Hospital, Taoyuan; 7 Department of Pathology, School of Medicine, Chang Gung University, Taoyuan; and 8 Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan The histo-blood group i and I antigens have been characterized as straight and branched repeats of N-acetyllactosamine, respectively, and the conversion of the straight-chain i to the branched-chain I structure on red cells is regulated to occur after birth. It has been demonstrated that the human I locus expresses 3 IGnT transcripts, IGnTA, IGnTB, and IGnTC, and that the last of these is responsible for the I branching formation on red cells. In the present investigation, the K-562 cell line was used as a model to show that the i-to-I transition in erythroid differentiation is determined by the transcription factor CCAAT/enhancer binding protein (C/EBP), which enhances transcription of the IGnTC gene, consequently leading to formation of the I antigen. Further investigation suggested that C/EBP IGnTC-activation activity is modulated at a posttranslational level, and that the phosphorylation status of C/EBP may have a crucial effect. Results from studies using adult and cord erythropoietic cells agreed with those derived using the K-562 cell model, with lentiviral expression of C/EBP in CD34+ hemopoietic cells demonstrating the determining role of C/EBP in the induction of the IGnTC gene as well as in I antigen expression. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. L. Yamamoto, T. A. Clark, S. L. Gee, J.-A. Kang, A. C. Schweitzer, A. Wickrema, and J. G. Conboy Alternative pre-mRNA splicing switches modulate gene expression in late erythropoiesis Blood, April 2, 2009; 113(14): 3363 - 3370. [Abstract] [Full Text] [PDF]

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