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Blood, 1 August 2007, Vol. 110, No. 3, pp. 1048-1054. Prepublished online as a Blood First Edition Paper on April 13, 2007; DOI 10.1182/blood-2006-11-057471. This Article Full Text Full Text (PDF) Supplemental Methods and Figures All Versions of this Article: blood-2006-11-057471v1 110/3/1048    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Murphy, A. T. Articles by Wroblewski, V. J. Search for Related Content PubMed PubMed Citation Articles by Murphy, A. T. Articles by Wroblewski, V. J. Related Collections Red Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  RED CELLS Quantitation of hepcidin from human and mouse serum using liquid chromatography tandem mass spectrometry Anthony T. Murphy1, Derrick R. Witcher2, Peng Luan2, and Victor J. Wroblewski1 Departments of1 Drug Disposition Bioproducts and 2 Biotechnology Discovery Research, Lilly Research Laboratories, Lilly Corporate Center, Indianapolis, IN The hepatic peptide hormone hepcidin is considered the central regulator of iron metabolism. Characterizing the circulating levels of this peptide is critical to understanding its role in the development of clinically relevant syndromes, such as anemia of inflammation/chronic disease, and may provide insight into potential clinical interventions. While quantitative methods have been published for the determination of urinary hepcidin and serum prohepcidin, no definitive methods have been published for the determination of hepcidin in serum. In this report, we describe a quantitative method for the determination of both human and mouse hepcidin in serum and plasma. The method employs protein precipitation and solid-phase extraction followed by liquid chromatographic separation and tandem mass spectrometry detection. The method has a quantitative range of 0.25 ng/mL to 500 ng/mL serum for mouse hepcidin and 1 ng/mL to 500 ng/mL serum for human hepcidin. The method uses small sample volumes (50 µL for mice and 100 µL for humans) and 96-well formats for rapid sample processing. The method was used to establish baseline serum and plasma concentrations of hepcidin in normal C57Bl/6 mice and healthy human volunteers. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: L. Ukarma, H. Johannes, U. Beyer, M. Zaug, B. Osterwalder, and A. Scherhag Hepcidin as a Predictor of Response to Epoetin Therapy in Anemic Cancer Patients Clin. Chem., July 1, 2009; 55(7): 1354 - 1360. [Abstract] [Full Text] [PDF] J. Malyszko Hepcidin Assays: Ironing Out Some Details Clin. J. Am. Soc. Nephrol., June 1, 2009; 4(6): 1015 - 1016. [Full Text] [PDF] I. Theurl, E. Aigner, M. Theurl, M. Nairz, M. Seifert, A. Schroll, T. Sonnweber, L. Eberwein, D. R. Witcher, A. T. Murphy, et al. Regulation of iron homeostasis in anemia of chronic disease and iron deficiency anemia: diagnostic and therapeutic implications Blood, May 21, 2009; 113(21): 5277 - 5286. [Abstract] [Full Text] [PDF] M. F Young, R. P Glahn, M. Ariza-Nieto, J. Inglis, G. Olbina, M. Westerman, and K. O O'Brien Serum hepcidin is significantly associated with iron absorption from food and supplemental sources in healthy young women Am. J. Clinical Nutrition, February 1, 2009; 89(2): 533 - 538. [Abstract] [Full Text] [PDF] D. M Frazer and G. J Anderson Hepcidin compared with prohepcidin: an absorbing story Am. J. Clinical Nutrition, February 1, 2009; 89(2): 475 - 476. [Full Text] [PDF] D. W. Swinkels and J. F. M. Wetzels Hepcidin: a new tool in the management of anaemia in patients with chronic kidney disease? Nephrol. Dial. Transplant., May 21, 2008; (2008) gfn267v1. [Full Text] [PDF]

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