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 Blood, 1 August 2007, Vol. 110, No. 3, pp. 806-814.
Prepublished online as a Blood First Edition Paper on April 5, 2007; DOI 10.1182/blood-2006-08-019190.

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REVIEW IN TRANSLATIONAL HEMATOLOGY

Endothelial potential of human embryonic stem cells

Shulamit Levenberg1, Janet Zoldan1, Yaara Basevitch1,2, and Robert Langer3

1 Department of Biomedical Engineering and 2 Biotechnology Interdisciplinary Unit, Technion-Israel Institute of Technology, Haifa, Israel; 3 Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge

Growing interest in using endothelial cells for therapeutic purposes has led to exploring human embryonic stem cells as a potential source for endothelial progenitor cells. Embryonic stem cells are advantageous when compared with other endothelial cell origins, due to their high proliferation capability, pluripotency, and low immunogenity. However, there are many challenges and obstacles to overcome before the vision of using embryonic endothelial progenitor cells in the clinic can be realized. Among these obstacles is the development of a productive method of isolating endothelial cells from human embryonic stem cells and elucidating their differentiation pathway. This review will focus on the endothelial potential of human embryonic stem cells that is described in current studies, with respect to the differentiation of human embryonic stem cells to endothelial cells, their isolation, and their characterization.


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