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Blood, 15 September 2007, Vol. 110, No. 6, pp. 1806-1813. Prepublished online as a Blood First Edition Paper on May 25, 2007; DOI 10.1182/blood-2007-02-075382. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2007-02-075382v1 110/6/1806    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Shepherd, B. E. Articles by Abkowitz, J. L. Search for Related Content PubMed PubMed Citation Articles by Shepherd, B. E. Articles by Abkowitz, J. L. Related Collections Stem Cells in Hematology Hematopoiesis and Stem Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Hematopoietic stem-cell behavior in nonhuman primates Bryan E. Shepherd1, Hans-Peter Kiem2, Peter M. Lansdorp3,4, Cynthia E. Dunbar5, Geraldine Aubert3,4, Andre LaRochelle5, Ruth Seggewiss5, Peter Guttorp6, and Janis L. Abkowitz7 1 Department of Biostatistics, Vanderbilt University, Nashville, TN; 2 Fred Hutchinson Cancer Research Institute, Seattle, WA; 3 Terry Fox Laboratory, British Columbia Cancer Agency, 4 Department of Medicine, University of British Columbia, Vancouver, Canada; 5 National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD; Departments of6 Statistics and 7 Medicine/Hematology, University of Washington, Seattle Little is known about the behavior of hematopoietic stem cells (HSCs) in primates because direct observations and competitive-repopulation assays are not feasible. Therefore, we used 2 different and independent experimental strategies, the tracking of transgene expression after retroviral-mediated gene transfer (N = 11 baboons; N = 7 rhesus macaques) and quantitation of the average telomere length of granulocytes (N = 132 baboons; N = 14 macaques), together with stochastic methods, to study HSC kinetics in vivo. The average replication rate for baboon HSCs is once per 36 weeks according to gene-marking analyses and once per 23 weeks according to telomere-shortening analyses. Comparable results were derived from the macaque data. These rates are substantially slower than the average replication rates previously reported for HSCs in mice (once per 2.5 weeks) and cats (once per 8.3 weeks). Because baboons and macaques live for 25 to 45 years, much longer than mice (2 years) and cats (12-18 years), we can compute that HSCs undergo a relatively constant number (80-200) of lifetime replications. Thus, our data suggest that the self-renewal capacity of mammalian stem cells in vivo is defined and evolutionarily conserved. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. W. Nienhuis Development of gene therapy for blood disorders Blood, May 1, 2008; 111(9): 4431 - 4444. [Abstract] [Full Text] [PDF] P. M. Lansdorp Telomeres, stem cells, and hematology Blood, February 15, 2008; 111(4): 1759 - 1766. [Abstract] [Full Text] [PDF]

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