SEARCH:   Advanced

Blood, 15 September 2007, Vol. 110, No. 6, pp. 1960-1969. Prepublished online as a Blood First Edition Paper on May 17, 2007; DOI 10.1182/blood-2006-09-045807. This Article Full Text Full Text (PDF) Supplemental Methods and Figures All Versions of this Article: blood-2006-09-045807v1 110/6/1960    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Cassese, G. Articles by Di Rosa, F. Search for Related Content PubMed PubMed Citation Articles by Cassese, G. Articles by Di Rosa, F. Related Collections Immunobiology Signal Transduction Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Bone marrow CD8 cells down-modulate membrane IL-7R expression and exhibit increased STAT-5 and p38 MAPK phosphorylation in the organ environment. Giuliana Cassese1, Elisabetta Parretta2, Laura Pisapia1, Angela Santoni2,4, John Guardiola1, and Francesca Di Rosa1,3 1 Institute of Genetics and Biophysics "Adriano Buzzati-Traverso," Consiglio Nazionale delle Ricerche, Naples; 2 Department of Experimental Medicine, Istituto Pasteur-Fondazione Cenci Bolognetti, University of Rome La Sapienza, Rome; 3 Institute of Molecular Biology and Pathology, Consiglio Nazionale delle Ricerche; 4 Regina Elena Cancer Institute, Centro Ricerca Sperimentale, Rome, Italy By comparing mature CD8-cell turnover in different organs, we previously demonstrated that CD8 cells proliferate predominantly in the bone marrow (BM). To investigate the mechanisms underlying such increased turnover, we compared BM, lymph nodes, and spleen CD8 cells from untreated C57BL/6 mice regarding in vivo proliferation within the organ; in vitro response to interleukin-7 (IL-7), IL-15, IL-21; ex vivo expression of membrane CD127 (IL-7R), intracellular Bcl-2, phospho–STAT-5 (signal transducer and activator of transcription 5), phospho-p38 mitogen activated protein kinase (MAPK); and in vivo proliferation on adoptive transfer. In the BM, the proliferation rate was increased for either total CD8 cells or individual CD44 and CD122 subsets. In contrast, purified CD8+ cells from the BM did not show an enhanced in vitro proliferative response to IL-7, IL-15, and IL-21 compared with corresponding spleen cells. After transfer and polyinosinic-polycytidylic acid (polyI:C) treatment, both spleen-derived and BM-derived CD8 cells from congenic donors proliferated approximately twice more in the recipient BM than in spleen and lymph nodes. Our results suggest that BM CD8 cells are not committed to self-renewal, but rather are stimulated in the organ. Molecular events constantly induced in the CD8 cells within the BM of untreated mice include increase of both phosphorylated STAT-5 and phosphorylated p38 intracellular levels, and the reduction of CD127 membrane expression. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: E. Parretta, G. Cassese, A. Santoni, J. Guardiola, A. Vecchio, and F. Di Rosa Kinetics of In Vivo Proliferation and Death of Memory and Naive CD8 T Cells: Parameter Estimation Based on 5-Bromo-2'-Deoxyuridine Incorporation in Spleen, Lymph Nodes, and Bone Marrow J. Immunol., June 1, 2008; 180(11): 7230 - 7239. [Abstract] [Full Text] [PDF]

	Copyright © 2007 by American Society of Hematology         Online ISSN: 1528-0020