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Blood, 1 October 2007, Vol. 110, No. 7, pp. 2351-2360. Prepublished online as a Blood First Edition Paper on July 10, 2007; DOI 10.1182/blood-2007-01-069914. This Article Full Text Full Text (PDF) Supplemental Methods and Figures All Versions of this Article: blood-2007-01-069914v1 110/7/2351    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Perry, S. S. Articles by Sun, X.-H. Search for Related Content PubMed PubMed Citation Articles by Perry, S. S. Articles by Sun, X.-H. Related Collections Hematopoiesis and Stem Cells Stem Cells in Hematology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Id1, but not Id3, directs long-term repopulating hematopoietic stem-cell maintenance S. Scott Perry1, Ying Zhao1, Lei Nie1, Shawn W. Cochrane1, Zhong Huang1, and Xiao-Hong Sun1 1 Immunobiology and Cancer Research Program, Oklahoma Medical Research Foundation, Oklahoma City E-proteins are widely expressed basic helix-loop-helix (HLH) transcription factors that regulate differentiation in many cell lineages, including lymphoid, muscle, and neuronal cells. E-protein function is controlled by HLH inhibitors such as Id and SCL/TAL1 proteins, which recently have been suggested to play a role in hematopoietic stem cell (HSC) differentiation. However, the precise stages when these proteins are expressed and their specific functions are not entirely clear. Using a knock-in mouse model where the sequence for the enhanced green fluorescent protein (GFP) was inserted downstream of the Id1 promoter, we were able to track Id1 expression on an individual cell basis and detected Id1 expression in long-term repopulating HSCs (LT-HSCs). Functional assays showed that the Id1/GFP+Lin–Sca1+c-kitHi population was highly enriched for LT-HSCs. Consistent with this expression pattern, Id1 deficiency led to a 2-fold reduction in the number of LT-HSCs defined as Lin–Sca1+c-kitHiCD48–CD150+. Primary bone marrow transplantation studies revealed that Id1 is dispensable for short-term engraftment. In contrast, both Id1–/– whole bone marrow and Lin–Sca1+c-kitHiThy1.1Lo-enriched HSCs, but not Id3–/– marrow, displayed impaired engraftment relative to wild-type controls in secondary transplantation assays. These findings suggest a unique role for Id1 in LT-HSC maintenance and hematopoietic development. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. W. Cochrane, Y. Zhao, R. S. Welner, and X.-H. Sun Balance between Id and E proteins regulates myeloid-versus-lymphoid lineage decisions Blood, January 29, 2009; 113(5): 1016 - 1026. [Abstract] [Full Text] [PDF] W. F. Tam, T.-L. Gu, J. Chen, B. H. Lee, L. Bullinger, S. Frohling, A. Wang, S. Monti, T. R. Golub, and D. G. Gilliland Id1 is a common downstream target of oncogenic tyrosine kinases in leukemic cells Blood, September 1, 2008; 112(5): 1981 - 1992. [Abstract] [Full Text] [PDF] L. J. Russell, T. Akasaka, A. Majid, K.-j. Sugimoto, E. Loraine Karran, I. Nagel, L. Harder, A. Claviez, S. Gesk, A. V. Moorman, et al. t(6;14)(p22;q32): a new recurrent IGH@ translocation involving ID4 in B-cell precursor acute lymphoblastic leukemia (BCP-ALL) Blood, January 1, 2008; 111(1): 387 - 391. [Abstract] [Full Text] [PDF]

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