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Blood, 1 October 2007, Vol. 110, No. 7, pp. 2432-2439. Prepublished online as a Blood First Edition Paper on July 2, 2007; DOI 10.1182/blood-2007-02-069997. This Article Full Text Full Text (PDF) Supplemental Figure All Versions of this Article: blood-2007-02-069997v1 110/7/2432    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lacroix, R. Articles by Dignat-George, F. Search for Related Content PubMed PubMed Citation Articles by Lacroix, R. Articles by Dignat-George, F. Related Collections Hematopoiesis and Stem Cells Hemostasis, Thrombosis, and Vascular Biology Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Activation of plasminogen into plasmin at the surface of endothelial microparticles: a mechanism that modulates angiogenic properties of endothelial progenitor cells in vitro Romaric Lacroix1, Florence Sabatier1, Agnès Mialhe1, Agnès Basire1, Ralph Pannell2, Hélène Borghi3, Stephane Robert1, Edouard Lamy1, Laurent Plawinski4, Laurence Camoin-Jau1, Victor Gurewich2, Eduardo Angles-Cano4, and Françoise Dignat-George1 1 Unité Mixte de Recherche S 608 (UMR-S 608), Institut National de la Santé et de la Recherche Médicale (INSERM), Université de la Méditerranée, Unité de Formation et de Recherche (UFR) de Pharmacie, Marseille, France; 2 Vascular Research Laboratory, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA; 3 Service de Microscopie Electronique, UFR de Médecine, Université de la Méditerranée, Marseille, France; 4 INSERM, Cyceron, Université de Caen, Caen, France The regulation of plasmin generation on cell surfaces is of critical importance in the control of vascular homeostasis. Cell-derived microparticles participate in the dissemination of biological activities. However, their capacity to promote plasmin generation has not been documented. In this study, we show that endothelial microparticles (EMPs) from tumor necrosis factor (TNF)–stimulated endothelial cells served as a surface for the generation of plasmin. The generation of plasmin involved expression of urokinase-type plasminogen activator (uPA) and its receptor (uPAR) at the surface of EMPs and was further increased by their ability to bind exogenous uPA on uPAR. Plasminogen was activated at the surface of EMPs in a dose-dependent, saturable, and specific manner as indicated by the inhibition of plasmin formation by -amino-caproic acid (-ACA) and carboxypeptidase B. EMP-induced plasmin generation affects tube formation mediated by endothelial progenitor cells. However, low amounts of EMPs increased tube formation, whereas higher concentrations inhibited it. Prevention of these effects by inhibitors of either uPA or plasmin underscore the key role of EMP-induced plasmin generation. In conclusion, we demonstrated that EMPs act as vectors supporting efficient plasmin generation and dissemination, a new pathway in the regulation of endothelial proteolytic activities with potential involvement in inflammation, angiogenesis, and atherosclerosis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: It's not size, it's substance Edward F. Plow and Elzbieta Pluskota Blood 2007 110: 2224-2225. [Full Text] [PDF] This article has been cited by other articles: F. Angelot, E. Seilles, S. Biichle, Y. Berda, B. Gaugler, J. Plumas, L. Chaperot, F. Dignat-George, P. Tiberghien, P. Saas, et al. Endothelial cell-derived microparticles induce plasmacytoid dendritic cell maturation: potential implications in inflammatory diseases Haematologica, November 1, 2009; 94(11): 1502 - 1512. [Abstract] [Full Text] [PDF] G. M. Thomas, L. Panicot-Dubois, R. Lacroix, F. Dignat-George, D. Lombardo, and C. Dubois Cancer cell-derived microparticles bearing P-selectin glycoprotein ligand 1 accelerate thrombus formation in vivo J. Exp. Med., August 31, 2009; 206(9): 1913 - 1927. [Abstract] [Full Text] [PDF] A. S. Leroyer, T. G. Ebrahimian, C. Cochain, A. Recalde, O. Blanc-Brude, B. Mees, J. Vilar, A. Tedgui, B. I. Levy, G. Chimini, et al. Microparticles From Ischemic Muscle Promotes Postnatal Vasculogenesis Circulation, June 2, 2009; 119(21): 2808 - 2817. [Abstract] [Full Text] [PDF] L. S. Barcelos, C. Duplaa, N. Krankel, G. Graiani, G. Invernici, R. Katare, M. Siragusa, M. Meloni, I. Campesi, M. Monica, et al. Human CD133+ Progenitor Cells Promote the Healing of Diabetic Ischemic Ulcers by Paracrine Stimulation of Angiogenesis and Activation of Wnt Signaling Circ. Res., May 8, 2009; 104(9): 1095 - 1102. [Abstract] [Full Text] [PDF] S. Simoncini, M.-S. Njock, S. Robert, L. Camoin-Jau, J. Sampol, J.-R. Harle, C. Nguyen, F. Dignat-George, and F. Anfosso TRAIL/Apo2L Mediates the Release of Procoagulant Endothelial Microparticles Induced by Thrombin In Vitro: A Potential Mechanism Linking Inflammation and Coagulation Circ. Res., April 24, 2009; 104(8): 943 - 951. [Abstract] [Full Text] [PDF] E. Pluskota, N. M. Woody, D. Szpak, C. M. Ballantyne, D. A. Soloviev, D. I. Simon, and E. F. Plow Expression, activation, and function of integrin {alpha}M{beta}2 (Mac-1) on neutrophil-derived microparticles Blood, September 15, 2008; 112(6): 2327 - 2335. [Abstract] [Full Text] [PDF]

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