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Blood, 1 October 2007, Vol. 110, No. 7, pp. 2520-2527. Prepublished online as a Blood First Edition Paper on June 26, 2007; DOI 10.1182/blood-2007-03-081299. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2007-03-081299v1 110/7/2520    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Koguchi, Y. Articles by Parker, D. C. Search for Related Content PubMed PubMed Citation Articles by Koguchi, Y. Articles by Parker, D. C. Related Collections Immunobiology Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Preformed CD40 ligand exists in secretory lysosomes in effector and memory CD4+ T cells and is quickly expressed on the cell surface in an antigen-specific manner Yoshinobu Koguchi1, Timothy J. Thauland1, Mark K. Slifka2, and David C. Parker1 1 Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland; 2 Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton CD40 ligand (CD40L) is an essential effector cytokine for macrophage activation, dendritic cell licensing, and T-cell–dependent antibody responses. Although CD40L is known to be made de novo following antigen recognition, several reports have described surface mobilization of preformed, intracellular CD40L in certain CD4+ effector T cells. Here we show that rapid surface expression of preformed CD40L following antigen recognition is a general property of both effector and memory CD4+ T cells, including in vitro and in vivo activated T-cell–receptor transgenic T cells, memory phenotype CD4+ T cells from pathogen-free naive mice, and polyclonal virus–specific effector and memory T cells. Intracellular CD40L is stored in secretory lysosomes, and colocalizes more strongly with Fas ligand than with CTLA-4, two other molecules that are delivered to the cell surface following antigen recognition. Stimulated surface expression of preformed CD40L is found in memory CD4+ T cells from CD40-deficient mice, indicating that it does not depend on CD40-induced internalization for delivery to the secretory compartment. We suggest that delivery of preformed CD40L to antigen-presenting cells (APCs) could enable antigen-specific activation of APCs in transient interactions that are too brief to permit de novo synthesis of CD40L. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: An adaptive route to innate immunity? Richard S. Kornbluth Blood 2007 110: 2221-2222. [Full Text] [PDF] This article has been cited by other articles: T. J. Thauland, Y. Koguchi, S. A. Wetzel, M. L. Dustin, and D. C. Parker Th1 and Th2 Cells Form Morphologically Distinct Immunological Synapses J. Immunol., July 1, 2008; 181(1): 393 - 399. [Abstract] [Full Text] [PDF] S. K. Chang, S. A. Mihalcik, and D. F. Jelinek B Lymphocyte Stimulator Regulates Adaptive Immune Responses by Directly Promoting Dendritic Cell Maturation J. Immunol., June 1, 2008; 180(11): 7394 - 7403. [Abstract] [Full Text] [PDF]

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