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Blood, 1 November 2007, Vol. 110, No. 9, pp. 3438-3446. Prepublished online as a Blood First Edition Paper on May 24, 2007; DOI 10.1182/blood-2006-11-055566. This Article Full Text Full Text (PDF) Supplemental Methods, Tables, and Figures Supplemental Figures and Videos All Versions of this Article: blood-2006-11-055566v1 110/9/3438    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Beltrami, A. P. Articles by Beltrami, C. A. Search for Related Content PubMed PubMed Citation Articles by Beltrami, A. P. Articles by Beltrami, C. A. Related Collections Hematopoiesis and Stem Cells Transplantation Free Research Articles Stem Cells in Hematology Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  STEM CELLS IN HEMATOLOGY Multipotent cells can be generated in vitro from several adult human organs (heart, liver, and bone marrow) Antonio P. Beltrami1, Daniela Cesselli1, Natascha Bergamin1, Patrizia Marcon1, Silvia Rigo1, Elisa Puppato1, Federica D'Aurizio1, Roberto Verardo2, Silvano Piazza2, Angela Pignatelli3, Alessandra Poz1, Umberto Baccarani1, Daniela Damiani1, Renato Fanin1, Laura Mariuzzi1, Nicoletta Finato1, Paola Masolini1, Silvia Burelli1, Ottorino Belluzzi3, Claudio Schneider2, and Carlo A. Beltrami1 1 Centro Interdipartimentale Medicina Rigenerativa (CIME), University of Udine, Udine; 2 Laboratorio Internazionale Consorzio Interuniversitario Biotecnologie (LNCIB), Trieste; and 3 Dipartimento Biologia, Sezione di Fisiologia e Biofisica–Centro di Neuroscienze, Universita di Ferrara, Ferrara, Italy The aims of our study were to verify whether it was possible to generate in vitro, from different adult human tissues, a population of cells that behaved, in culture, as multipotent stem cells and if these latter shared common properties. To this purpose, we grew and cloned finite cell lines obtained from adult human liver, heart, and bone marrow and named them human multipotent adult stem cells (hMASCs). Cloned hMASCs, obtained from the 3 different tissues, expressed the pluripotent state–specific transcription factors Oct-4, NANOG, and REX1, displayed telomerase activity, and exhibited a wide range of differentiation potential, as shown both at a morphologic and functional level. hMASCs maintained a human diploid DNA content, and shared a common gene expression signature, compared with several somatic cell lines and irrespectively of the tissue of isolation. In particular, the pathways regulating stem cell self-renewal/maintenance, such as Wnt, Hedgehog, and Notch, were transcriptionally active. Our findings demonstrate that we have optimized an in vitro protocol to generate and expand cells from multiple organs that could be induced to acquire morphologic and functional features of mature cells even embryologically not related to the tissue of origin. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Life in plastic is fantastic Paolo Bianco Blood 2007 110: 3090. [Full Text] [PDF] This article has been cited by other articles: L. S. Hart and W. S. El-Deiry Invincible, but Not Invisible: Imaging Approaches Toward In Vivo Detection of Cancer Stem Cells J. Clin. Oncol., June 10, 2008; 26(17): 2901 - 2910. [Abstract] [Full Text] [PDF] C. A. Beltrami Life is plastic Blood, March 1, 2008; 111(5): 2942 - 2943. [Full Text] [PDF]

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