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Blood, 1 November 2007, Vol. 110, No. 9, pp. 3472-3479. Prepublished online as a Blood First Edition Paper on July 26, 2007; DOI 10.1182/blood-2007-06-095414. This Article Full Text Full Text (PDF) Supplemental Methods and Figure All Versions of this Article: blood-2007-06-095414v1 110/9/3472    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Klenovsek, K. Articles by Mach, M. Search for Related Content PubMed PubMed Citation Articles by Klenovsek, K. Articles by Mach, M. Related Collections Immunobiology Transplantation Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  TRANSPLANTATION Protection from CMV infection in immunodeficient hosts by adoptive transfer of memory B cells Karin Klenovsek1, Florian Weisel2, Andrea Schneider2, Uwe Appelt3, Stipan Jonjic4, Martin Messerle5, Birgit Bradel-Tretheway6, Thomas H. Winkler2, and Michael Mach1 1 Institute for Clinical and Molecular Virology, University Hospital Erlangen, 2 Institute for Biology, Chair of Genetics, Hematopoesis Unit, and 3 Nikolaus-Fiebiger-Center for Molecular Medicine, University Erlangen-Nuremberg, Erlangen, Germany; 4 Department of Histology and Embryology, Faculty of Medicine, University of Rijeka, Rijeka, Croatia; 5 Hannover Medical School, Hannover, Germany; and 6 University of Rochester Medical Center, Rochester, NY Severe disease associated with cytomegalovirus (CMV) infection is still a major problem in patients who undergo transplantation. Support of the patients' immune defense against the virus is a major goal in transplantation medicine. We have used the murine model of CMV (MCMV) to investigate the potential of a cell-based strategy to support the humoral antiviral immune response. Immunocompetent C57BL/6 mice were infected with MCMV, and memory B cells from the immune animals were adoptively transferred into T-cell– and B-cell–deficient RAG-1–/– mice. Following MCMV infection, a virus-specific IgG response developed within 4 to 7 days in the recipient animals. Concomitantly, a significant reduction in viral titers and DNA copies in several organs was observed. In addition, the memory B-cell transfer provided long-term protection from the lethal course of the infection that is invariably seen in immunodeficient animals. Transfer of memory B cells was also effective in protecting from an already ongoing viral infection, indicating a therapeutic potential of virus-specific memory B cells. T cells were not involved in this process. Our data provide evidence that a cell-based strategy to support the humoral immune response can be effective to combat infectious pathogens in severely immunodeficient hosts. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. M. Walton, P. Wyrsch, M. W. Munks, A. Zimmermann, H. Hengel, A. B. Hill, and A. Oxenius The Dynamics of Mouse Cytomegalovirus-Specific CD4 T Cell Responses during Acute and Latent Infection J. Immunol., July 15, 2008; 181(2): 1128 - 1134. [Abstract] [Full Text] [PDF]

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