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Blood, 1 January 2008, Vol. 111, No. 1, pp. 142-149. Prepublished online as a Blood First Edition Paper on September 28, 2007; DOI 10.1182/blood-2007-07-102558. This Article Full Text Full Text (PDF) Supplemental Figure All Versions of this Article: blood-2007-07-102558v1 111/1/142    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Jeannet, G. Articles by Held, W. Search for Related Content PubMed PubMed Citation Articles by Jeannet, G. Articles by Held, W. Related Collections Hematopoiesis and Stem Cells Signal Transduction Gene Expression Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Long-term, multilineage hematopoiesis occurs in the combined absence of β-catenin and -catenin Grégoire Jeannet1, Marina Scheller2, Léonardo Scarpellino1, Stéphane Duboux3, Noemie Gardiol1, Jonathan Back1, Fabien Kuttler3, Ilaria Malanchi3, Walter Birchmeier2, Achim Leutz2, Joerg Huelsken3, and Werner Held1 1 Ludwig Institute for Cancer Research, Lausanne Branch, University of Lausanne, Lausanne, Switzerland; 2 Max-Delbrück Center for Molecular Medicine, Berlin, Germany; and 3 Ecole Polytechnique Fédérale de Lausanne, Swiss Institute for Experimental Cancer Research, Lausanne, Switzerland The canonical Wnt signaling pathway plays key roles in stem-cell maintenance, progenitor cell expansion, and lineage decisions. Transcriptional responses induced by Wnt depend on the association of either β-catenin or -catenin with lymphoid enhancer factor/T cell factor transcription factors. Here we show that hematopoiesis, including thymopoiesis, is normal in the combined absence of β- and -catenin. Double-deficient hematopoietic stem cells maintain long-term repopulation capacity and multilineage differentiation potential. Unexpectedly, 2 independent ex vivo reporter gene assays show that Wnt signal transmission is maintained in double-deficient hematopoietic stem cells, thymocytes, or peripheral T cells. In contrast, Wnt signaling is strongly reduced in thymocytes lacking TCF-1 or in nonhematopoietic cells devoid of β-catenin. These data provide the first evidence that hematopoietic cells can transduce canonical Wnt signals in the combined absence of β- and -catenin. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D. Kovalovsky, Y. Yu, M. Dose, A. Emmanouilidou, T. Konstantinou, K. Germar, K. Aghajani, Z. Guo, M. Mandal, and F. Gounari {beta}-Catenin/Tcf Determines the Outcome of Thymic Selection in Response to {alpha}{beta}TCR Signaling J. Immunol., September 15, 2009; 183(6): 3873 - 3884. [Abstract] [Full Text] [PDF] M. Xu, A. Sharma, D. L. Wiest, and J. M. Sen Pre-TCR-Induced {beta}-Catenin Facilitates Traversal through {beta}-Selection J. Immunol., January 15, 2009; 182(2): 751 - 758. [Abstract] [Full Text] [PDF] T. C. Luis, F. Weerkamp, B. A. E. Naber, M. R. M. Baert, E. F. E. de Haas, T. Nikolic, S. Heuvelmans, R. R. De Krijger, J. J. M. van Dongen, and F. J. T. Staal Wnt3a deficiency irreversibly impairs hematopoietic stem cell self-renewal and leads to defects in progenitor cell differentiation Blood, January 15, 2009; 113(3): 546 - 554. [Abstract] [Full Text] [PDF] S. Malhotra, Y. Baba, K. P. Garrett, F. J. T. Staal, R. Gerstein, and P. W. Kincade Contrasting Responses of Lymphoid Progenitors to Canonical and Noncanonical Wnt Signals J. Immunol., September 15, 2008; 181(6): 3955 - 3964. [Abstract] [Full Text] [PDF] T. Grigoryan, P. Wend, A. Klaus, and W. Birchmeier Deciphering the function of canonical Wnt signals in development and disease: conditional loss- and gain-of-function mutations of {beta}-catenin in mice Genes & Dev., September 1, 2008; 22(17): 2308 - 2341. [Abstract] [Full Text] [PDF]

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