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Blood, 1 January 2008, Vol. 111, No. 1, pp. 150-159. Prepublished online as a Blood First Edition Paper on October 4, 2007; DOI 10.1182/blood-2007-05-089292. This Article Full Text Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2007-05-089292v1 111/1/150    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Fancke, B. Articles by O'Keeffe, M. Search for Related Content PubMed PubMed Citation Articles by Fancke, B. Articles by O'Keeffe, M. Related Collections Hematopoiesis and Stem Cells Immunobiology Chemokines, Cytokines, and Interleukins Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS M-CSF: a novel plasmacytoid and conventional dendritic cell poietin Ben Fancke1, Mark Suter1,2, Hubertus Hochrein1, and Meredith O'Keeffe1 1 Research Department, Bavarian Nordic GmbH, Martinsried, Germany; and 2 Institute of Virology, University of Zurich, Zurich, Switzerland The critical importance of plasmacytoid dendritic cells (pDCs) in viral infection, autoimmunity, and tolerance has focused major attention on these cells that are rare in blood and immune organs of humans and mice. The recent development of an Flt-3 ligand (FL) culture system of bone marrow cells has led to the simple generation of large numbers of pDCs that resemble their in vivo steady-state counterparts. The FL system has allowed unforeseen insight into the biology of pDCs, and it is assumed that FL is the crucial growth factor for these cells. Surprisingly we have found that a cell type with high capacity for interferon- (IFN-) production in response to CpG-containing oligonucleotides, a feature of pDCs, develop within macrophage–colony-stimulating factor (M-CSF)–generated bone marrow cultures. Analysis of this phenomenon revealed that M-CSF is able to drive pDCs as well as conventional DCs (cDCs) from BM precursor cells in vitro. Furthermore, application of M-CSF to mice was able to drive pDCs and cDCs development in vivo. It is noteworthy that using mice deficient in FL indicated that the M-CSF-driven generation of pDCs and cDCs in vitro and in vivo was independent of endogenous FL. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D. Kingston, M. A. Schmid, N. Onai, A. Obata-Onai, D. Baumjohann, and M. G. Manz The concerted action of GM-CSF and Flt3-ligand on in vivo dendritic cell homeostasis Blood, July 23, 2009; 114(4): 835 - 843. [Abstract] [Full Text] [PDF] M. Merad and M. G. Manz Dendritic cell homeostasis Blood, April 9, 2009; 113(15): 3418 - 3427. [Abstract] [Full Text] [PDF] T. Banovic, K. A. Markey, R. D. Kuns, S. D. Olver, N. C. Raffelt, A. L. Don, M. A. Degli-Esposti, C. R. Engwerda, K. P. A. MacDonald, and G. R. Hill Graft-versus-Host Disease Prevents the Maturation of Plasmacytoid Dendritic Cells J. Immunol., January 15, 2009; 182(2): 912 - 920. [Abstract] [Full Text] [PDF] D. A. Hume Macrophages as APC and the Dendritic Cell Myth J. Immunol., November 1, 2008; 181(9): 5829 - 5835. [Abstract] [Full Text] [PDF]

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