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Blood, 15 May 2008, Vol. 111, No. 10, pp. 4854-4861. Prepublished online as a Blood First Edition Paper on February 13, 2008; DOI 10.1182/blood-2007-08-105247. This Article Full Text Full Text (PDF) Supplemental Videos All Versions of this Article: blood-2007-08-105247v1 111/10/4854    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Chernysh, I. N. Articles by Weisel, J. W. Search for Related Content PubMed PubMed Citation Articles by Chernysh, I. N. Articles by Weisel, J. W. Related Collections Hemostasis, Thrombosis, and Vascular Biology Plenary Papers Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  PLENARY PAPER Dynamic imaging of fibrin network formation correlated with other measures of polymerization Irina N. Chernysh1, and John W. Weisel1 1 Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia Using deconvolution microscopy, we visualized in real time fibrin network formation in the hydrated state. Individual mobile fibers were observed before the gel point determined by eye. After gelation, an initial fibrin network was seen, which evolved over time by addition of new fibers and elongation and branching of others. Furthermore, some fibers in the network moved for a time. We quantified network formation by number of branch points, and longitudinal and lateral growth of fibers. Eighty percent of branch points were formed, and 70% of all fibers reached their maximum length at the gel point. In contrast, at the gel point, fiber diameter, measured as fluorescence intensity, was less than 25% and turbidity was less than 15% of the maximum values of the fully formed clot. The cumulative percentage of fibers reaching their final length and the number of branch points attained maximum values at 60% of maximum turbidity. Lateral fiber growth reached a plateau at the same time as turbidity. Measurements of clot mechanical properties revealed that the clots achieved maximum stiffness and minimum plasticity after the structural parameters reached their maxima. These results provide new information on the relative time sequence of events during fibrin network formation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Fibrin formation on fast forward Mattia Rocco Blood 2008 111: 4839. [Full Text] [PDF] This article has been cited by other articles: E. T. O'Brien III, M. R. Falvo, D. Millard, B. Eastwood, R. M. Taylor II, and R. Superfine Ultrathin self-assembled fibrin sheets PNAS, December 9, 2008; 105(49): 19438 - 19443. [Abstract] [Full Text] [PDF] R. A. Campbell, K. A. Overmyer, C. R. Bagnell, and A. S. Wolberg Cellular Procoagulant Activity Dictates Clot Structure and Stability as a Function of Distance From the Cell Surface Arterioscler Thromb Vasc Biol, December 1, 2008; 28(12): 2247 - 2254. [Abstract] [Full Text] [PDF]

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