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Blood, 15 June 2008, Vol. 111, No. 12, pp. 5562-5570. Prepublished online as a Blood First Edition Paper on April 18, 2008; DOI 10.1182/blood-2007-11-126219. This Article Full Text Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2007-11-126219v1 111/12/5562    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Karsunky, H. Articles by Weissman, I. L. Search for Related Content PubMed PubMed Citation Articles by Karsunky, H. Articles by Weissman, I. L. Related Collections Hematopoiesis and Stem Cells Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS AND STEM CELLS Flk2+ common lymphoid progenitors possess equivalent differentiation potential for the B and T lineages Holger Karsunky1, Matthew A. Inlay1, Thomas Serwold1, Deepta Bhattacharya1, and Irving L. Weissman1 1 Institute of Stem Cell Biology and Regenerative Medicine at Stanford University, Departments of Pathology and Developmental Biology, Stanford University School of Medicine, CA Mature blood cells develop from multipotent hematopoietic stem cells through a series of sequential intermediates in which the developmental potential for particular blood lineages is progressively extinguished. We previously reported the identification of one of these developmental intermediates, the common lymphoid progenitor (CLP), which can give rise to T cells, B cells, dendritic cells (DCs), and natural killer cells (NKs), but lacks myeloid and erythroid potential. Recently, several studies have suggested that the T-cell and DC potential of CLP is limited or absent, and/or that CLP contains significant myeloid potential. Here, we show that the originally identified CLP population can be divided into functionally distinct subsets based on the expression of the tyrosine kinase receptor, Flk2. The Flk2+ subset contains robust in vivo and in vitro T-cell, B-cell, DC, and NK potential, but lacks myeloid potential and, therefore, represents an oligopotent, lymphoid-restricted progenitor. This population of cells does not appear to be B cell–biased and robustly reconstitutes both B and T lineages in vivo, consistent with its being a physiologic progenitor of both of these subsets. Thus, Flk2 expression defines a homogeneous, readily obtainable subset of bone marrow CLP that is completely lymphoid-committed and can differentiate equivalently well into both B and T lineages. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. Cheng, H. N. Charoudeh, P. Brodin, Y. Tang, T. Lakshmikanth, P. Hoglund, S. E. W. Jacobsen, and E. Sitnicka Distinct and Overlapping Patterns of Cytokine Regulation of Thymic and Bone Marrow-Derived NK Cell Development J. Immunol., February 1, 2009; 182(3): 1460 - 1468. [Abstract] [Full Text] [PDF] K. D. Bunting CLPs find their inner T-cell potential Blood, January 22, 2009; 113(4): 766 - 767. [Full Text] [PDF] T. Serwold, L. I. Richie Ehrlich, and I. L. Weissman Reductive isolation from bone marrow and blood implicates common lymphoid progenitors as the major source of thymopoiesis Blood, January 22, 2009; 113(4): 807 - 815. [Abstract] [Full Text] [PDF]

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