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Blood, 15 June 2008, Vol. 111, No. 12, pp. 5621-5628. Prepublished online as a Blood First Edition Paper on April 1, 2008; DOI 10.1182/blood-2008-01-134155. This Article Full Text Full Text (PDF) Supplemental Methods and Figures All Versions of this Article: blood-2008-01-134155v1 111/12/5621    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Aucher, A. Articles by Hudrisier, D. Search for Related Content PubMed PubMed Citation Articles by Aucher, A. Articles by Hudrisier, D. Related Collections Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Capture of plasma membrane fragments from target cells by trogocytosis requires signaling in T cells but not in B cells Anne Aucher1, Eddy Magdeleine1, Etienne Joly1, and Denis Hudrisier1,2 1 Institut de Pharmacologie et de Biologie Structurale (IPBS), Centre National de Recherche Scientifique (CNRS), Unité Mixte de Recherche 5089, Toulouse; and 2 Université Paul Sabatier, Toulouse, France Upon recognition of their respective cellular partners, T and B cells acquire their antigens by a process of membrane capture called trogocytosis. Here, we report that various inhibitors of actin polymerization or of kinases involved in intracellular signaling partially or fully inhibited trogocytosis by CD8+ and CD4+ T cells, whereas they had no effect on trogocytosis by B cells. Similarly, trogocytosis by T cells was inhibited at 4°C, whereas in B cells it was independent of temperature, indicating that trogocytosis by B cells does not rely on active processes. By contrast, most inhibitors we tested impaired both T-cell and B-cell activation. The differential effect of inhibitors on T-cell and B-cell trogocytosis was not due to the higher affinity of the B-cell receptor for its cognate antigen compared with the affinity of the T-cell receptor for its own antigen, but it correlated tightly with the abilities of T cells and B cells to form conjugates with their target cells in the presence of inhibitors. Trogocytosis thus has different requirements in different cell types. Moreover, the capture of membrane antigen by B cells is identified as a novel signaling-independent event of B-cell biology. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

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