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Blood, 1 February 2008, Vol. 111, No. 3, pp. 1334-1343. Prepublished online as a Blood First Edition Paper on October 17, 2007; DOI 10.1182/blood-2007-07-101907. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2007-07-101907v1 111/3/1334    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bhaduri-McIntosh, S. Articles by Miller, G. Search for Related Content PubMed PubMed Citation Articles by Bhaduri-McIntosh, S. Articles by Miller, G. Related Collections Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Repertoire and frequency of immune cells reactive to Epstein-Barr virus–derived autologous lymphoblastoid cell lines Sumita Bhaduri-McIntosh1, Marisa J. Rotenberg1, Benjamin Gardner1, Marie Robert2, and George Miller3 1 Department of Pediatrics, Yale University School of Medicine, New Haven, CT; 2 Pediatric and Adolescent Medicine of Orange, CT; and 3 Departments of Pediatrics, Molecular Biophysics and Biochemistry, and Epidemiology and Public Health, Yale University School of Medicine, New Haven, CT Answers to questions about frequency and repertoire of immune cells, relative contributions made by different types of immune cells toward the total Epstein-Barr virus (EBV)–directed response and the variation of such responses in healthy persons have been elusive because of disparities in assays, antigen presenting cells, and antigenic sources used in previous experiments. In this study, we addressed these questions using an assay that allowed direct comparison of responses generated by different types of cells of the immune system. This short-term (20-hour) ex vivo assay measured interferon- production by blood cells in response to autologous EBV-transformed lymphoblastoid cell lines (LCLs). Our experiments defined the variation in responses among persons and clearly distinguished 10 healthy EBV-immune from 10 healthy EBV-naive persons. In EBV-immune persons, 33% of responding cells were CD4+, 43.3% were CD8+, and 12.9% were - T cells. LCL-reactive CD8+ T cells were only 1.7-fold more frequent than similarly reactive CD4+T cells. Responses by - T cells were 6-fold higher in seropositive than in seronegative persons. Our findings emphasize the importance of CD4+ and - T-cell responses and have implications for immunotherapy and for identifying defects in T-cell populations that might predispose to development of EBV-associated lymphomas. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. L. Gulley and W. Tang Laboratory Assays for Epstein-Barr Virus-Related Disease J. Mol. Diagn., July 1, 2008; 10(4): 279 - 292. [Abstract] [Full Text] [PDF]

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