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 Blood, 15 February 2008, Vol. 111, No. 4, pp. 1876-1884.
Prepublished online as a Blood First Edition Paper on November 21, 2007; DOI 10.1182/blood-2007-06-093609.

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HEMATOPOIESIS

Targeting a GFP reporter gene to the MIXL1 locus of human embryonic stem cells identifies human primitive streak–like cells and enables isolation of primitive hematopoietic precursors

Richard P. Davis1, Elizabeth S. Ng1, Magdaline Costa1, Anna K. Mossman1, Koula Sourris1, Andrew G. Elefanty1, and Edouard G. Stanley1

1 Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Australia

Differentiating human embryonic stem cells (HESCs) represent an experimental platform for establishing the relationships between the earliest lineages that emerge during human development. Here we report the targeted insertion in HESCs of sequences encoding green fluorescent protein (GFP) into the locus of MIXL1, a gene transiently expressed in the primitive streak during embryogenesis.1,2 GFP fluorescence in MIXL1GFP/w HESCs differentiated in the presence of BMP4 reported the expression of MIXL1, permitting the identification of viable human primitive streak-like cells. The use of GFP as a reporter for MIXL1 combined with cell surface staining for platelet-derived growth factor receptor alpha (PDGFR{alpha}) enabled the isolation of a cell population that was highly enriched in primitive hematopoietic precursors, the earliest derivatives of the primitive streak. These experiments demonstrate the utility of MIXL1GFP/w HESCs for analyzing the previously inaccessible events surrounding the development of human primitive streak-like cells and their subsequent commitment to hematopoiesis.


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