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Blood, 15 February 2008, Vol. 111, No. 4, pp. 1876-1884. Prepublished online as a Blood First Edition Paper on November 21, 2007; DOI 10.1182/blood-2007-06-093609. This Article Full Text Full Text (PDF) Supplemental Tables and Figures All Versions of this Article: blood-2007-06-093609v1 111/4/1876    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Davis, R. P. Articles by Stanley, E. G. Search for Related Content PubMed PubMed Citation Articles by Davis, R. P. Articles by Stanley, E. G. Related Collections Hematopoiesis and Stem Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Targeting a GFP reporter gene to the MIXL1 locus of human embryonic stem cells identifies human primitive streak–like cells and enables isolation of primitive hematopoietic precursors Richard P. Davis1, Elizabeth S. Ng1, Magdaline Costa1, Anna K. Mossman1, Koula Sourris1, Andrew G. Elefanty1, and Edouard G. Stanley1 1 Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Australia Differentiating human embryonic stem cells (HESCs) represent an experimental platform for establishing the relationships between the earliest lineages that emerge during human development. Here we report the targeted insertion in HESCs of sequences encoding green fluorescent protein (GFP) into the locus of MIXL1, a gene transiently expressed in the primitive streak during embryogenesis.1,2 GFP fluorescence in MIXL1GFP/w HESCs differentiated in the presence of BMP4 reported the expression of MIXL1, permitting the identification of viable human primitive streak-like cells. The use of GFP as a reporter for MIXL1 combined with cell surface staining for platelet-derived growth factor receptor alpha (PDGFR) enabled the isolation of a cell population that was highly enriched in primitive hematopoietic precursors, the earliest derivatives of the primitive streak. These experiments demonstrate the utility of MIXL1GFP/w HESCs for analyzing the previously inaccessible events surrounding the development of human primitive streak-like cells and their subsequent commitment to hematopoiesis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: F. Timmermans, I. Velghe, L. Vanwalleghem, M. De Smedt, S. Van Coppernolle, T. Taghon, H. D. Moore, G. Leclercq, A. W. Langerak, T. Kerre, et al. Generation of T Cells from Human Embryonic Stem Cell-Derived Hematopoietic Zones J. Immunol., June 1, 2009; 182(11): 6879 - 6888. [Abstract] [Full Text] [PDF] S. Irion, M.C. Nostro, S.J. Kattman, and G.M. Keller Directed Differentiation of Pluripotent Stem Cells: From Developmental Biology to Therapeutic Applications Cold Spring Harb Symp Quant Biol, March 27, 2009; (2009) sqb.2008.73.065v1. [Abstract] [PDF] K. Suzuki, K. Mitsui, E. Aizawa, K. Hasegawa, E. Kawase, T. Yamagishi, Y. Shimizu, H. Suemori, N. Nakatsuji, and K. Mitani Highly efficient transient gene expression and gene targeting in primate embryonic stem cells with helper-dependent adenoviral vectors PNAS, September 16, 2008; 105(37): 13781 - 13786. [Abstract] [Full Text] [PDF]

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