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Blood, 15 February 2008, Vol. 111, No. 4, pp. 1924-1932. Prepublished online as a Blood First Edition Paper on December 6, 2007; DOI 10.1182/blood-2007-08-104489. This Article Full Text Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2007-08-104489v1 111/4/1924    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sugiyama, D. Articles by Nakano, T. Search for Related Content PubMed PubMed Citation Articles by Sugiyama, D. Articles by Nakano, T. Related Collections Hematopoiesis and Stem Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Differential context-dependent effects of friend of GATA-1 (FOG-1) on mast-cell development and differentiation Daijiro Sugiyama1, Makoto Tanaka1, Kenji Kitajima1, Jie Zheng1, Hilo Yen1, Tomotaka Murotani2, Atsushi Yamatodani2, and Toru Nakano1 1 Department of Stem Cell Pathology, Medical School, and Graduate School of Frontier Biosciences and 2 Department of Medical Physics and Engineering, Division of Health Sciences, Graduate School of Medicine, Osaka University, Japan Friend of GATA-1 (FOG-1) is a binding partner of GATA-1, a zinc finger transcription factor with crucial roles in erythroid, megakaryocytic, and mast-cell differentiation. FOG-1 is indispensable for the function of GATA-1 during erythro/megakaryopoiesis, but FOG-1 is not expressed in mast cells. Here, we analyzed the role of FOG-1 in mast-cell differentiation using a combined experimental system with conditional gene expression and in vitro hematopoietic induction of mouse embryonic stem cells. Expression of FOG-1 during the progenitor period inhibited the differentiation of mast cells and enhanced the differentiation of neutrophils. Analysis using a mutant of PU.1, a transcription factor that positively or negatively cooperates with GATA-1, revealed that this lineage skewing was caused by disrupted binding between GATA-1 and PU.1, which is a prerequisite for mast-cell differentiation. However, FOG-1 expression in mature mast cells brought approximately a reversible loss of the mast-cell phenotype. In contrast to the lineage skewing, the loss of the mast-cell phenotype was caused by down-regulation of MITF, a basic helix-loop-helix transcription factor required for mast-cell differentiation and maturation. These results indicate that FOG-1 inhibits mast-cell differentiation in a differentiation stage-dependent manner, and its effects are produced via different molecular mechanisms. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: Z. Huang, L. C. Dore, Z. Li, S. H. Orkin, G. Feng, S. Lin, and J. D. Crispino GATA-2 Reinforces Megakaryocyte Development in the Absence of GATA-1 Mol. Cell. Biol., September 15, 2009; 29(18): 5168 - 5180. [Abstract] [Full Text] [PDF] S. T. Chou, E. Khandros, L. C. Bailey, K. E. Nichols, C. R. Vakoc, Y. Yao, Z. Huang, J. D. Crispino, R. C. Hardison, G. A. Blobel, et al. Graded repression of PU.1/Sfpi1 gene transcription by GATA factors regulates hematopoietic cell fate Blood, July 30, 2009; 114(5): 983 - 994. [Abstract] [Full Text] [PDF] J. T. Dobson, J. Seibert, E. M. Teh, S. Da'as, R. B. Fraser, B. H. Paw, T.-J. Lin, and J. N. Berman Carboxypeptidase A5 identifies a novel mast cell lineage in the zebrafish providing new insight into mast cell fate determination Blood, October 1, 2008; 112(7): 2969 - 2972. [Abstract] [Full Text] [PDF]

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