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Blood, 15 February 2008, Vol. 111, No. 4, pp. 2444-2451. Prepublished online as a Blood First Edition Paper on November 30, 2007; DOI 10.1182/blood-2007-09-115006. This Article Full Text Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2007-09-115006v1 111/4/2444    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Weksberg, D. C. Articles by Goodell, M. A. Search for Related Content PubMed PubMed Citation Articles by Weksberg, D. C. Articles by Goodell, M. A. Related Collections Stem Cells in Hematology Related Articles in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  STEM CELLS IN HEMATOLOGY CD150– side population cells represent a functionally distinct population of long-term hematopoietic stem cells David C. Weksberg1,2, Stuart M. Chambers2,3, Nathan C. Boles2,3, and Margaret A. Goodell13 1 Department of Molecular and Human Genetics; 2 Stem Cells and Regenerative Medicine Center; and 3 Interdepartmental Program in Cell and Molecular Biology, Baylor College of Medicine, Houston, TX Hematopoietic stem cells (HSCs) are a self-renewing population of bone marrow cells that replenish the cellular elements of blood throughout life. HSCs represent a paradigm for the study of stem-cell biology, because robust methods for prospective isolation of HSCs have facilitated rigorous characterization of these cells. Recently, a new isolation method was reported, using the SLAM family of cell-surface markers, including CD150 (SlamF1), to offer potential advantages over established protocols. We examined the overlap between SLAM family member expression with an established isolation scheme based on Hoechst dye efflux (side population; SP) in conjunction with canonical HSC cell-surface markers (Sca-1, c-Kit, and lineage markers). Importantly, we find that stringent gating of SLAM markers is essential to achieving purity in HSC isolation and that the inclusion of canonical HSC markers in the SLAM scheme can greatly augment HSC purity. Furthermore, we observe that both CD150+ and CD150– cells can be found within the SP population and that both populations can contribute to long-term multilineage reconstitution. Thus, using SLAM family markers to isolate HSCs excludes a substantial fraction of the marrow HSC compartment. Interestingly, these 2 subpopulations are functionally distinct, with respect to lineage output as well as proliferative status. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Articles in Blood Online: CD150– cells are transiently reconstituting multipotent progenitors with little or no stem cell activity Mark J. Kiel, Omer H. Yilmaz, and Sean J. Morrison Blood 2008 111: 4413-4414. [Full Text] [PDF] Response: The CD150high compartment is not the exclusive reservoir of LT-HSCs within the bone marrow Stuart M. Chambers, David C. Weksberg, and Margaret A. Goodell Blood 2008 111: 4414-4415. [Full Text] [PDF] This article has been cited by other articles: D. G. Kent, M. R. Copley, C. Benz, S. Wohrer, B. J. Dykstra, E. Ma, J. Cheyne, Y. Zhao, M. B. Bowie, Y. Zhao, et al. Prospective isolation and molecular characterization of hematopoietic stem cells with durable self-renewal potential Blood, June 18, 2009; 113(25): 6342 - 6350. [Abstract] [Full Text] [PDF] A. J. Simonnet, J. Nehme, P. Vaigot, V. Barroca, P. Leboulch, and D. Tronik-Le Roux Phenotypic and Functional Changes Induced in Hematopoietic Stem/Progenitor Cells After Gamma-Ray Radiation Exposure Stem Cells, June 1, 2009; 27(6): 1400 - 1409. [Abstract] [Full Text] [PDF] F. Cerisoli, L. Cassinelli, G. Lamorte, S. Citterio, F. Bertolotti, M. C. Magli, and S. Ottolenghi Green fluorescent protein transgene driven by Kit regulatory sequences is expressed in hematopoietic stem cells Haematologica, March 1, 2009; 94(3): 318 - 325. [Abstract] [Full Text] [PDF] J. Tolar, A. Ishida-Yamamoto, M. Riddle, R. T. McElmurry, M. Osborn, L. Xia, T. Lund, C. Slattery, J. Uitto, A. M. Christiano, et al. Amelioration of epidermolysis bullosa by transfer of wild-type bone marrow cells Blood, January 29, 2009; 113(5): 1167 - 1174. [Abstract] [Full Text] [PDF] M. J. Kiel, O. H. Yilmaz, and S. J. Morrison CD150- cells are transiently reconstituting multipotent progenitors with little or no stem cell activity Blood, April 15, 2008; 111(8): 4413 - 4414. [Full Text] [PDF] S. M. Chambers, D. C. Weksberg, and M. A. Goodell Response: The CD150high compartment is not the exclusive reservoir of LT-HSCs within the bone marrow Blood, April 15, 2008; 111(8): 4414 - 4415. [Full Text] [PDF]

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