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Blood, 1 March 2008, Vol. 111, No. 5, pp. 2631-2635. Prepublished online as a Blood First Edition Paper on December 17, 2007; DOI 10.1182/blood-2007-07-099622. This Article Full Text Full Text (PDF) Supplemental Methods and Tables All Versions of this Article: blood-2007-07-099622v1 111/5/2631    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Google Scholar Articles by Delorme, B. Articles by Charbord, P. PubMed PubMed Citation Articles by Delorme, B. Articles by Charbord, P. Related Collections Brief Reports Hematopoiesis and Stem Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS AND STEM CELLS Brief Report Specific plasma membrane protein phenotype of culture-amplified and native human bone marrow mesenchymal stem cells Bruno Delorme1, Jochen Ringe2, Nathalie Gallay1, Yves Le Vern3, Dominique Kerboeuf3, Christian Jorgensen4, Philippe Rosset5, Luc Sensebé1,6, Pierre Layrolle7, Thomas Häupl2, and Pierre Charbord1 1 Inserm, Equipe ESPRI/EA-3855, University François Rabelais, Faculty of Medicine, Tours, France; 2 Department of Rheumatology and Clinical Immunology, Laboratory for tissue engineering, Charité-University Medicine, Berlin, Germany; 3 Flow cytometry laboratory, Research Unit for Animal Infectiology and Public Health, Institut National de Recherche Agronomique, Nouzilly, France; 4 Inserm, U844, Lapeyronie Hospital, Montpellier, France; 5 Department of Orthopedic Surgery, University Hospital, Tours, France; 6 Etablissement Français du Sang-Centre-Atlantique, Tours, France; and 7 Inserm, U791, Faculty of Dental Surgery, Nantes, France We have studied the plasma membrane protein phenotype of human culture-amplified and native bone marrow mesenchymal stem cells (BM MSCs). We have found, using microarrays and flow cytometry, that cultured cells express specifically 113 transcripts and 17 proteins that were not detected in hematopoietic cells. These antigens define a lineage-homogenous cell population of mesenchymal cells, clearly distinct from the hematopoietic lineages, and distinguishable from other cultured skeletal mesenchymal cells (periosteal cells and synovial fibroblasts). Among the specific membrane proteins present on cultured MSCs, 9 allowed the isolation from BM mononuclear cells of a minute population of native MSCs. The enrichment in colony-forming units–fibroblasts was low for CD49b, CD90, and CD105, but high for CD73, CD130, CD146, CD200, and integrin alphaV/beta5. In addition, the expression of CD73, CD146, and CD200 was down-regulated in differentiated cells. The new marker CD200, because of its specificity and immunomodulatory properties, deserves further in-depth studies. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

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