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 Blood, 1 March 2008, Vol. 111, No. 5, pp. 2636-2639.
Prepublished online as a Blood First Edition Paper on January 3, 2008; DOI 10.1182/blood-2007-11-124685.

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HEMATOPOIESIS AND STEM CELLS

Brief Report

Primitive erythropoiesis and megakaryopoiesis in the yolk sac are independent of c-myb

Joanna Tober1, Kathleen E. McGrath1,2, and James Palis1,2

1 Center for Pediatric Biomedical Research and 2 Department of Pediatrics, University of Rochester Medical Center, NY

Hematopoiesis initiates within the yolk sac of mammalian embryos in overlapping primitive and definitive waves, each containing erythroid and megakaryocyte progenitors. c-myb–null mouse fetuses lack definitive erythrocytes but contain primitive erythroblasts and hepatic megakaryocytes. However, it is unclear if c-myb–null embryos harbor definitive erythroid or any megakaryocyte progenitors. We determined that c-myb was not expressed in primitive erythroid precursors and that c-myb–null embryos had normal primitive erythroid and megakaryocyte progenitor numbers and kinetics between embryonic day (E) 7.0 and E9.0. While primitive hematopoiesis is c-myb–independent, no definitive erythroid potential was detected in c-myb–null embryos, confirming that definitive erythropoiesis, beginning at E8.25 in the yolk sac, is completely c-myb–dependent. In contrast, reduced numbers of megakaryocyte progenitors with restricted proliferative capacity persist in E10.5 yolk sac and E11.5 liver. Despite this impaired megakaryocyte potential, c-myb–null fetuses had normal platelet numbers at E12.5 but became thrombocytopenic by E15.5, suggesting that c-myb is required for sustained thrombopoiesis.


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