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Blood, 15 March 2008, Vol. 111, No. 6, pp. 3249-3256. Prepublished online as a Blood First Edition Paper on December 20, 2007; DOI 10.1182/blood-2007-06-097295. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2007-06-097295v1 111/6/3249    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wandall, H. H. Articles by Slichter, S. J. Search for Related Content PubMed PubMed Citation Articles by Wandall, H. H. Articles by Slichter, S. J. Related Collections Transfusion Medicine Free Research Articles Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  TRANSFUSION MEDICINE Galactosylation does not prevent the rapid clearance of long-term, 4°C-stored platelets Hans H. Wandall13, Karin M. Hoffmeister2, Anne Louise Sørensen13, Viktoria Rumjantseva2, Henrik Clausen13, John H. Hartwig2, and Sherrill J. Slichter4 1 ZymeQuest, Beverly, MA; 2 Division of Hematology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA; 3 Department of Cellular and Molecular Medicine, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark; and 4 Puget Sound Blood Center and University of Washington School of Medicine, Seattle Cold storage of platelets for transfusion is desirable to extend platelet storage times and to prevent bacterial growth. However, the rapid clearance of cold-stored platelets prevents their use. A novel method for preventing the rapid clearance of cold-stored platelets has previously been developed in a murine model. Cold storage induces the clustering and recognition of exposed β-N-acetylglucosamine (βGlcNAc) on platelet surfaces. Glycosylation of βGlcNAc residues with uridine 5'-diphosphogalactose (UDP-galactose) results in the normal survival of short-term (2 h) 0°C-stored murine platelets. Based on this finding, we developed a similar glycosylation process by adding UDP-galactose to human apheresis platelets. A phase 1 clinical trial was conducted transfusing radiolabeled autologous apheresis platelets stored for 48 hours at 4°C with or without pretreatment with UDP-galactose. In contrast to the murine study, galactosylation of human platelets did not prevent the accelerated platelet clearance routinely observed after 4°C storage. We next developed a murine model of platelet storage for 48 hours at 4°C and showed that UDP-galactose treatment of murine platelets also did not prevent their rapid clearance, in agreement with the human platelet study. We conclude that different mechanisms of clearance may exist for short- and long-term cold-stored platelets. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Platelets come back in from the cold Miguel Lozano Blood 2008 111: 2951. [Full Text] [PDF] This article has been cited by other articles: A. L. Sorensen, V. Rumjantseva, S. Nayeb-Hashemi, H. Clausen, J. H. Hartwig, H. H. Wandall, and K. M. Hoffmeister Role of sialic acid for platelet life span: exposure of {beta}-galactose results in the rapid clearance of platelets from the circulation by asialoglycoprotein receptor-expressing liver macrophages and hepatocytes Blood, August 20, 2009; 114(8): 1645 - 1654. [Abstract] [Full Text] [PDF]

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