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Blood, 1 April 2008, Vol. 111, No. 7, pp. 3684-3691. Prepublished online as a Blood First Edition Paper on February 6, 2008; DOI 10.1182/blood-2007-05-091728. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2007-05-091728v1 111/7/3684    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Elzey, B. D. Articles by Ratliff, T. L. Search for Related Content PubMed PubMed Citation Articles by Elzey, B. D. Articles by Ratliff, T. L. Related Collections Hemostasis, Thrombosis, and Vascular Biology Immunobiology Related Articles in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Platelet-derived CD154 enables T-cell priming and protection against Listeria monocytogenes challenge Bennett D. Elzey1,2, Nathan W. Schmidt3, Scott A. Crist1, Timothy P. Kresowik4, John T. Harty3,5, Bernhard Nieswandt6,7, and Timothy L. Ratliff1,8 1 Department of Comparative Pathobiology, Purdue University, West Lafayette, IN; 2 Department of Urology, Indiana University, Indianapolis; 3 Department of Microbiology, 4 Department of Urology, and 5 Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City; 6 Rudolf Virchow Center, DFG Research Center for Experimental Biomedicine, and 7 Institute of Clinical Biochemistry and Pathobiochemistry, University of Wurzburg, Wurzburg, Germany; and 8 Purdue Cancer Center, Purdue University, West Lafayette, IN Collagen exposure in tissue activates platelets, initiates wound healing, and modulates adaptive immunity. In this report, data are presented to demonstrate a requirement for platelet-derived CD154 for both collagen-induced augmentation of T-cell immunity and induction of pro-tective immunity to Listeria challenge. Specifically, we demonstrate that Ad5 encoding the membrane-bound form of ovalbumin (Ad5-mOVA) delivered in collagen induces higher ovalbumin-specific cytotoxic T lymphocyte (CTL) activity in a dose-dependent manner compared with Ad5-mOVA delivered in PBS. Increased CTL activity was dependent on the ability of platelets to respond to collagen and to express CD154. Furthermore, mice immunized with low-dose Ad5-mOVA in collagen were able to control a challenge of Listeria monocytogenes recombinant for ovalbumin expression (Lm-OVA), whereas mice immunized with low-dose Ad5-mOVA in PBS were not. These data indicate that in a physiologic setting that mimics wounding, platelets perform a sentinel function when antigen dose is too low to provoke an efficient immune response, and can enhance the generation of antigen-specific CD8 T cells that are functionally relevant to the host. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Articles in Blood Online: Platelet components associated with acute transfusion reactions: the role of platelet-derived soluble CD40 ligand Fabrice Cognasse, Jean Marc Payrat, Larry Corash, Jean Claude Osselaer, and Olivier Garraud Blood 2008 112: 4779-4780. [Full Text] [PDF] Response: Are PDMVs the biologically active source of CD154 in ATR? Bennett D. Elzey, Scott A. Crist, Daniel L. Sprague, and Timothy L. Ratliff Blood 2008 112: 4780-4781. [Full Text] [PDF] This article has been cited by other articles: J. Yin and T. A. Ferguson Identification of an IFN-{gamma}-Producing Neutrophil Early in the Response to Listeria monocytogenes J. Immunol., June 1, 2009; 182(11): 7069 - 7073. [Abstract] [Full Text] [PDF] F. Cognasse, J. M. Payrat, L. Corash, J. C. Osselaer, and O. Garraud Platelet components associated with acute transfusion reactions: the role of platelet-derived soluble CD40 ligand Blood, December 1, 2008; 112(12): 4779 - 4780. [Full Text] [PDF] B. D. Elzey, S. A. Crist, D. L. Sprague, and T. L. Ratliff Response: Are PDMVs the biologically active source of CD154 in ATR? Blood, December 1, 2008; 112(12): 4780 - 4781. [Full Text] [PDF]

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