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Blood, 1 April 2008, Vol. 111, No. 7, pp. 3778-3792. Prepublished online as a Blood First Edition Paper on January 24, 2008; DOI 10.1182/blood-2007-10-117531. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2007-10-117531v1 111/7/3778    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Marafioti, T. Articles by Mason, D. Y. Search for Related Content PubMed PubMed Citation Articles by Marafioti, T. Articles by Mason, D. Y. Related Collections Neoplasia Phagocytes Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  NEOPLASIA Novel markers of normal and neoplastic human plasmacytoid dendritic cells Teresa Marafioti1, Jennifer C. Paterson1, Erica Ballabio1, Kaaren K. Reichard2, Sara Tedoldi1, Kevin Hollowood3, Michael Dictor4, Martin-Leo Hansmann5, Stefano A. Pileri6, Martin J. Dyer7, Silvano Sozzani8, Ivan Dikic9, Andrey S. Shaw10, Tony Petrella11, Harald Stein12, Peter G. Isaacson13, Fabio Facchetti8, and David Y. Mason1 1 Leukaemia Research Fund Immunodiagnostics Unit, Nuffield Department of Clinical Laboratory Sciences, John Radcliffe Hospital, Oxford, United Kingdom; 2 Department of Pathology, University of New Mexico, Albuquerque, NM; 3 Department of Cellular Pathology, John Radcliffe Hospital, Oxford, United Kingdom; 4 Department of Pathology, Lund University Hospital, Lund, Sweden; 5 Senckenberg Pathology Institute, Johann Wolfgang Goethe-University Clinic Frankfurt am Main, Frankfurt am Main, Germany; 6 Haematopathology Unit, "L&A Seragnoli" Institute of Haematology, University of Bologna, Bologna, Italy; 7 Medical Research Council Toxicology Unit, University of Leicester, Leicester, United Kingdom; 8 Department of Pathology and General Pathology, University of Brescia, Brescia, Italy; 9 Institute of Biochemistry II, Goethe University School of Medicine, University Clinic Frankfurt am Main, Frankfurt, Germany; 10 Department of Pathology, Washington University School of Medicine, St Louis, MO; 11 Department of Pathology and Centre of Pathology, Hospital-University Centre, Dijon, France; 12 Institute for Pathology, Campus Benjamin Franklin, Charité University Medicine Berlin, Berlin, Germany; and 13 Department of Pathology, University College London, London, United Kingdom Plasmacytoid dendritic cells (pDCs) are involved in innate immunity (eg, by secreting interferons) and also give rise to CD4+CD56+ hematodermic neoplasms. We report extensive characterization of human pDCs in routine tissue samples, documenting the expression of 19 immunohistologic markers, including signaling molecules (eg, BLNK), transcription factors (eg, ICSBP/IRF8 and PU.1), and Toll-like receptors (TLR7, TLR9). Many of these molecules are expressed in other cell types (principally B cells), but the adaptor protein CD2AP was essentially restricted to pDCs, and is therefore a novel immunohistologic marker for use in tissue biopsies. We found little evidence for activation-associated morphologic or phenotypic changes in conditions where pDCs are greatly increased (eg, Kikuchi disease). Most of the molecules were retained in the majority of pDC neoplasms, and 3 (BCL11A, CD2AP, and ICSBP/IRF8) were also commonly negative in leukemia cutis (acute myeloid leukemia in the skin), a tumor that may mimic pDC neoplasia. In summary, we have documented a range of molecules (notably those associated with B cells) expressed by pDCs in tissues and peripheral blood (where pDCs were detectable in cytospins at a frequency of < 1% of mononuclear cells) and also defined potential new markers (in particular CD2AP) for the diagnosis of pDC tumors. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. M. Imanguli, W. D. Swaim, S. C. League, R. E. Gress, S. Z. Pavletic, and F. T. Hakim Increased T-bet+ cytotoxic effectors and type I interferon-mediated processes in chronic graft-versus-host disease of the oral mucosa Blood, April 9, 2009; 113(15): 3620 - 3630. [Abstract] [Full Text] [PDF] M. Merad and M. G. Manz Dendritic cell homeostasis Blood, April 9, 2009; 113(15): 3418 - 3427. [Abstract] [Full Text] [PDF] B. Chetaille, F. Bertucci, P. Finetti, B. Esterni, A. Stamatoullas, J. M. Picquenot, M. C. Copin, F. Morschhauser, O. Casasnovas, T. Petrella, et al. Molecular profiling of classical Hodgkin lymphoma tissues uncovers variations in the tumor microenvironment and correlations with EBV infection and outcome Blood, March 19, 2009; 113(12): 2765 - 3775. [Abstract] [Full Text] [PDF] F. Facchetti, S. A. Pileri, C. Agostinelli, M. P. Martelli, M. Paulli, A. Venditti, M. F Martelli, and B. Falini Cytoplasmic nucleophosmin is not detected in blastic plasmacytoid dendritic cell neoplasm Haematologica, February 1, 2009; 94(2): 285 - 288. [Abstract] [Full Text] [PDF]

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