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 Blood, 15 April 2008, Vol. 111, No. 8, pp. 4092-4095.
Prepublished online as a Blood First Edition Paper on February 1, 2008; DOI 10.1182/blood-2007-11-122150.

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HEMATOPOIESIS AND STEM CELLS

Brief Report

Survivin overexpression alone does not alter megakaryocyte ploidy nor interfere with erythroid/megakaryocytic lineage development in transgenic mice

Donald J. McCrann1, Todd Yezefski2, Hao G. Nguyen1, Nicholas Papadantonakis1, Hui Liu2, Qiang Wen2, John D. Crispino2, and Katya Ravid1

1 Department of Biochemistry, Boston University School of Medicine, Boston, MA; and 2 Division of Hematology/Oncology; Department of Medicine, Northwestern University, Chicago, IL

The level of survivin was reported to be scarce in mouse megakaryocytes (MKs) compared with erythroid cells. Considering this finding and previously reported in vitro data showing decreased MK ploidy upon retroviral-mediated overexpression of survivin, we sought to examine whether ectopic survivin expression in the MK lineage might alter ploidy level in vivo. Here we report the generation of 2 tissue specific hematopoietic transgenic mouse models, one expressing survivin in both the erythroid and MK lineages and the other expressing survivin solely in the MK lineage. Survivin protein overexpression was confirmed in MKs and erythrocytes. Surprisingly, analysis of both transgenic mouse lines showed no detectable changes in MK number, ploidy level, and platelet and erythrocyte counts, as compared with control mice. We conclude that elevated survivin expression does not alter MK/erythroid lineage development and that elevated survivin, alone, does not interfere with MK ploidy in vivo.


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