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Blood, 1 May 2008, Vol. 111, No. 9, pp. 4653-4659. Prepublished online as a Blood First Edition Paper on March 3, 2008; DOI 10.1182/blood-2007-11-123844. This Article Full Text Full Text (PDF) Supplemental Tables and Figure All Versions of this Article: blood-2007-11-123844v1 111/9/4653    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Google Scholar Articles by Mamani-Matsuda, M. Articles by Weill, J.-C. PubMed PubMed Citation Articles by Mamani-Matsuda, M. Articles by Weill, J.-C. Related Collections Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY The human spleen is a major reservoir for long-lived vaccinia virus–specific memory B cells Maria Mamani-Matsuda1, Antonio Cosma2,3, Sandra Weller1, Ahmad Faili1, Caroline Staib3,4, Loïc Garçon5, Olivier Hermine6, Odile Beyne-Rauzy7, Claire Fieschi8, Jacques-Olivier Pers9, Nina Arakelyan10, Bruno Varet6, Alain Sauvanet11, Anne Berger12, François Paye13, Jean-Marie Andrieu10, Marc Michel14, Bertrand Godeau14, Pierre Buffet15, Claude-Agnès Reynaud1, and Jean-Claude Weill1 1 Inserum U783, "Développement du Système Immunitaire," Université Paris Descartes, Faculté de Médecine, Site Necker-Enfants Malades, Paris, France; 2 Clinical cooperation group "Immune monitoring," Helmholtz Zentrum München-German Research Center for Environmental Health, Munich, Germany; 3 Institute of Molecular Virology and Clinical cooperation group "Antigen-specific immunotherapy," Helmhotz Zentrum München-German Research Center for Environmental Health, Munich, Germany; 4 Institute of Virology, Technical University, Munich, Germany; 5 Centre Hospitalier Universitaire (CHU) Bicêtre, Service d'Hématologie, Le Kremlin-Bicêtre, France; 6 Hôpital Necker-Enfants Malades, Service d'Hématologie adulte, Paris, France; 7 CHU de Toulouse, Service de Médecine Interne et Immunopathologie, Toulouse, France; 8 Hôpital Saint-Louis, Unité d'Immunopathologie, Paris, France; 9 CHU de Brest, Laboratoire d'Immunologie et Pathologie, Brest, France; 10 Hôpital Européen Georges Pompidou, Service de Cancérologie, Paris, France; 11 Hôpital Beaujon, Service de Chirurgie Digestive, Clichy, France; 12 Hôpital Européen Georges Pompidou, Service de Chirurgie Digestive, Paris, France; 13 Hôpital Saint Antoine, Service de Chirurgie Digestive, Paris, France; 14 CHU Henri Mondor, Service de Médecine Interne, Créteil, France; and 15 Centre Médical, Institut Pasteur, Paris, France The fact that you can vaccinate a child at 5 years of age and find lymphoid B cells and antibodies specific for this vaccination 70 years later remains an immunologic enigma. It has never been determined how these long-lived memory B cells are maintained and whether they are protected by storage in a special niche. We report that, whereas blood and spleen compartments present similar frequencies of IgG+ cells, antismallpox memory B cells are specifically enriched in the spleen where they account for 0.24% of all IgG+ cells (ie, 10-20 million cells) more than 30 years after vaccination. They represent, in contrast, only 0.07% of circulating IgG+ B cells in blood (ie, 50-100 000 cells). An analysis of patients either splenectomized or rituximab-treated confirmed that the spleen is a major reservoir for long-lived memory B cells. No significant correlation was observed between the abundance of these cells in blood and serum titers of antivaccinia virus antibodies in this study, including in the contrasted cases of B cell– depleting treatments. Altogether, these data provide evidence that in humans, the two arms of B-cell memory—long-lived memory B cells and plasma cells—have specific anatomic distributions—spleen and bone marrow—and homeostatic regulation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

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