Blood online
Home About Blood Authors Subscriptions Permission Advertising Public Access contact us
 

 
Advanced
Current Issue
First Edition
Archives
Submit to Blood
Search
American Society of Hematology
Meeting Abstracts
Email Alerts
 Blood, 1 July 2008, Vol. 112, No. 1, pp. 100-110.
Prepublished online as a Blood First Edition Paper on March 12, 2008; DOI 10.1182/blood-2007-07-104455.

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Figures and Videos
Right arrow All Versions of this Article:
blood-2007-07-104455v1
112/1/100    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Right arrow Rights and Permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Google Scholar
Right arrow Articles by Stepanova, V.
Right arrow Articles by Cines, D. B.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stepanova, V.
Right arrow Articles by Cines, D. B.
Related Collections
Right arrow Hemostasis, Thrombosis, and Vascular Biology
Right arrowRelated Article in Blood Online
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

arrow to previous article Previous Article  |  Table of Contents  |  Next Article next article arrow

HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY

Nuclear translocation of urokinase-type plasminogen activator

Victoria Stepanova1,2, Tatiana Lebedeva1, Alice Kuo1, Serge Yarovoi1, Sergei Tkachuk3, Sergei Zaitsev4, Khalil Bdeir1, Inna Dumler3, Michael S. Marks1, Yelena Parfyonova2, Vsevolod A. Tkachuk2, Abd Al-Roof Higazi1,5, and Douglas B. Cines1

1 Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia; 2 Russian Cardiology Research Center, Moscow, Russia; 3 Hannover Medical School, Hannover, Germany; 4 Department of Pharmacology, University of Pennsylvania, Philadelphia; and 5 Department of Clinical Biochemistry, Hadassah University Hospital and Hebrew University-Hadassah Medical School, Jerusalem, Israel

Urokinase-type plasminogen activator (uPA) participates in diverse (patho)physiological processes through intracellular signaling events that affect cell adhesion, migration, and proliferation, although the mechanisms by which these occur are only partially understood. Here we report that upon cell binding and internalization, single-chain uPA (scuPA) translocates to the nucleus within minutes. Nuclear translocation does not involve proteolytic activation or degradation of scuPA. Neither the urokinase receptor (uPAR) nor the low-density lipoprotein-related receptor (LRP) is required for nuclear targeting. Rather, translocation involves the binding of scuPA to the nucleocytoplasmic shuttle protein nucleolin through a region containing the kringle domain. RNA interference and mutational analysis demonstrate that nucleolin is required for the nuclear transport of scuPA. Furthermore, nucleolin is required for the induction smooth muscle {alpha}-actin ({alpha}-SMA) by scuPA. These data reveal a novel pathway by which uPA is rapidly translocated to the nucleus where it might participate in regulating gene expression.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related Article in Blood Online:

Urokinase: the identity crisis continues
Steven L. Gonias
Blood 2008 112: 8. [Full Text] [PDF]





click for free articles
home about blood authors subscriptions permissions advertising public access contact us
  Copyright © 2008 by American Society of Hematology         Online ISSN: 1528-0020