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Blood, 1 July 2008, Vol. 112, No. 1, pp. 120-130. Prepublished online as a Blood First Edition Paper on March 6, 2008; DOI 10.1182/blood-2007-09-114181. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2007-09-114181v1 112/1/120    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Liu, S. D. Articles by Miceli, M. C. Search for Related Content PubMed PubMed Citation Articles by Liu, S. D. Articles by Miceli, M. C. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Endogenous galectin-1 enforces class I–restricted TCR functional fate decisions in thymocytes Scot D. Liu1, Chan C. Whiting2, Tamar Tomassian3, Mabel Pang4, Stephanie J. Bissel1, Linda G. Baum4, Valeri V. Mossine5, Françoise Poirier6, Margaret E. Huflejt7, and M. Carrie Miceli1,3 1 Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles; 2 Ingenuity Systems, Partner & Professional Services, Redwood City, CA; 3 Molecular Biology Institute and 4 Department of Pathology and Laboratory Medicine, University of California, Los Angeles; 5 Department of Biochemistry, University of Missouri, Columbia; 6 Institut Jacques Monod, Centre National de la Recherche Scientifique Unité Mixte de Recherche Universities, Paris, France; and 7 GlycoMedical Research Institute, La Jolla, CA During thymocyte development, the T-cell receptor (TCR) can discriminate major histocompatibility complex (MHC)/peptide ligands over a narrow range of affinities and translate subtle differences into functional fate decisions. How small differences in TCR input are translated into absolute differences in functional output is unclear. We examined the effects of galectin-1 ablation in the context of class-I–restricted thymocyte development. Galectin-1 expression opposed TCR partial agonist-driven positive selection, but promoted TCR agonist-driven negative selection of conventional CD8+ T cells. Galectin-1 expression also promoted TCR agonist-driven CD8 intestinal intraepithelial lymphocytes (IEL) development. Recombinant galectin-1 enhanced TCR binding to agonist/MHC complexes and promoted a negative-selection-signaling signature, reflected in intensified rapid and transient extracellular signal-regulated kinase (ERK) activation. In contrast, galectin-1 expression antagonized ERK activity in thymocytes undergoing positive selection. We propose that galectin-1 aids in discriminating TCR-directed fate decisions by promoting TCR binding to agonist/MHC complexes and enforcing agonist-driven signals, while opposing partial-agonist signals. In this way, galectin-1 widens the distinction between TCR-directed functional fate cues. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Thymocyte-fate decisions: bittersweet new flavor Guy Werlen Blood 2008 112: 7-8. [Full Text] [PDF] This article has been cited by other articles: M. Pang, J. He, P. Johnson, and L. G. Baum CD45-Mediated Fodrin Cleavage during Galectin-1 T Cell Death Promotes Phagocytic Clearance of Dying Cells J. Immunol., June 1, 2009; 182(11): 7001 - 7008. [Abstract] [Full Text] [PDF] A. Norambuena, C. Metz, L. Vicuna, A. Silva, E. Pardo, C. Oyanadel, L. Massardo, A. Gonzalez, and A. Soza Galectin-8 Induces Apoptosis in Jurkat T Cells by Phosphatidic Acid-mediated ERK1/2 Activation Supported by Protein Kinase A Down-regulation J. Biol. Chem., May 8, 2009; 284(19): 12670 - 12679. [Abstract] [Full Text] [PDF] L. V Norling, M. Perretti, and D. Cooper Endogenous galectins and the control of the host inflammatory response J. Endocrinol., May 1, 2009; 201(2): 169 - 184. [Abstract] [Full Text] [PDF] S. D. Liu, T. Tomassian, K. W. Bruhn, J. F. Miller, F. Poirier, and M. C. Miceli Galectin-1 Tunes TCR Binding and Signal Transduction to Regulate CD8 Burst Size J. Immunol., May 1, 2009; 182(9): 5283 - 5295. [Abstract] [Full Text] [PDF]

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