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Blood, 15 November 2008, Vol. 112, No. 10, pp. 4227-4234. Prepublished online as a Blood First Edition Paper on August 29, 2008; DOI 10.1182/blood-2008-04-151498. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2008-04-151498v1 112/10/4227    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Orzechowska, B. U. Articles by Wong, S. W. Search for Related Content PubMed PubMed Citation Articles by Orzechowska, B. U. Articles by Wong, S. W. Related Collections Immunobiology Neoplasia Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  NEOPLASIA Rhesus macaque rhadinovirus-associated non-Hodgkin lymphoma: animal model for KSHV-associated malignancies Beata U. Orzechowska1,*, Michael F. Powers1,2,*, Jerald Sprague1, He Li1, Bonnie Yen1, Robert P. Searles1, Michael K. Axthelm1,2, and Scott W. Wong1,2,3 1 Vaccine and Gene Therapy Institute, Oregon Health & Science University, West Campus, Beaverton; 2 Division of Pathobiology and Immunology, Oregon National Primate Research Center, Beaverton; and 3 Department of Molecular Microbiology and Immunology, Oregon Health & Science University, Portland Rhesus macaque rhadinovirus (RRV) is closely related to Kaposi sarcoma-associated herpesvirus (KSHV) and is associated with the development of B-cell hyperplasia and persistent lymphadenopathy resembling multicentric Castleman disease in rhesus macaques (RMs) coinfected with simian immunodeficiency virus (SIV). Here we investigated whether RMs experimentally infected with SIV and RRV can develop other disease manifestations observed in HIV- and KSHV-infected patients. As reported earlier, inoculation of SIV-infected RMs with RRV results in persistent RRV infection, whereas immunocompetent animals infected with RRV exhibit viremia 2 weeks after infection, followed by a period of no virus detection until they are subsequently made immunodeficient by SIV infection. A subset of animals developed abnormal cellular proliferations characterized as extranodal lymphoma and a proliferative mesenchymal lesion. In situ hybridization and immunohistochemistry analysis indicate RRV is present in both malignancies, and DNA microarray analysis detected viral interleukin-6 (vIL-6) and viral FLICE-like inhibitory protein (vFLIP) transcripts. Reverse-transcriptase polymerase chain reaction analysis confirmed vIL-6 and vFLIP expression, and that of RRV open reading frames 72 and 73, homologs of KSHV open reading frames shown to be expressed in primary effusion lymphoma. These data support the utility of the RRV-/SIV-infected RM as an excellent animal model to investigate KSHV-like pathogenesis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: K. W. Wen, D. P. Dittmer, and B. Damania Disruption of LANA in Rhesus Rhadinovirus Generates a Highly Lytic Recombinant Virus J. Virol., October 1, 2009; 83(19): 9786 - 9802. [Abstract] [Full Text] [PDF] M. Okroj, L. Mark, A. Stokowska, S. W. Wong, N. Rose, D. J. Blackbourn, B. O. Villoutreix, O. B. Spiller, and A. M. Blom Characterization of the Complement Inhibitory Function of Rhesus Rhadinovirus Complement Control Protein (RCP) J. Biol. Chem., January 2, 2009; 284(1): 505 - 514. [Abstract] [Full Text] [PDF]

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