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Blood, 15 July 2008, Vol. 112, No. 2, pp. 295-307. Prepublished online as a Blood First Edition Paper on March 10, 2008; DOI 10.1182/blood-2007-07-103697. This Article Full Text Full Text (PDF) Supplemental Tables and Figures All Versions of this Article: blood-2007-07-103697v1 112/2/295    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ng, F. Articles by Tanavde, V. Search for Related Content PubMed PubMed Citation Articles by Ng, F. Articles by Tanavde, V. Related Collections Hematopoiesis and Stem Cells Chemokines, Cytokines, and Interleukins Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS AND STEM CELLS PDGF, TGF-β, and FGF signaling is important for differentiation and growth of mesenchymal stem cells (MSCs): transcriptional profiling can identify markers and signaling pathways important in differentiation of MSCs into adipogenic, chondrogenic, and osteogenic lineages Felicia Ng1,*, Shayne Boucher2, Susie Koh1, Konduru S. R. Sastry1, Lucas Chase2, Uma Lakshmipathy2, Cleo Choong3, Zheng Yang4, Mohan C. Vemuri2, Mahendra S. Rao2, and Vivek Tanavde1 1 Genome and Gene Expression Analysis Group, Bioinformatics Institute, Agency for Science Technology and Research (A*STAR), Singapore; 2 Stem Cells and Regenerative Medicine, Invitrogen Corporation, Carlsbad CA; 3 Laboratory of Stem Cell Biology, Singapore Stem Cell Consortium, Singapore; and 4 Department of Orthopaedic Surgery, Yong Loo Lin School of Medicine, Tissue Engineering Program, National University of Singapore, Singapore We compared the transcriptomes of marrow-derived mesenchymal stem cells (MSCs) with differentiated adipocytes, osteocytes, and chondrocytes derived from these MSCs. Using global gene-expression profiling arrays to detect RNA transcripts, we have identified markers that are specific for MSCs and their differentiated progeny. Further, we have also identified pathways that MSCs use to differentiate into adipogenic, chondrogenic, and osteogenic lineages. We identified activin-mediated transforming growth factor (TGF)–β signaling, platelet-derived growth factor (PDGF) signaling and fibroblast growth factor (FGF) signaling as the key pathways involved in MSC differentiation. The differentiation of MSCs into these lineages is affected when these pathways are perturbed by inhibitors of cell surface receptor function. Since growth and differentiation are tightly linked processes, we also examined the importance of these 3 pathways in MSC growth. These 3 pathways were necessary and sufficient for MSC growth. Inhibiting any of these pathways slowed MSC growth, whereas a combination of TGF-β, PDGF, and β-FGF was sufficient to grow MSCs in a serum-free medium up to 5 passages. Thus, this study illustrates it is possible to predict signaling pathways active in cellular differentiation and growth using microarray data and experimentally verify these predictions. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: A new window on MSCs Ernestina Schipani Blood 2008 112: 217-218. [Full Text] [PDF]

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