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Blood, 15 July 2008, Vol. 112, No. 2, pp. 350-361. Prepublished online as a Blood First Edition Paper on May 6, 2008; DOI 10.1182/blood-2007-12-128215. This Article Full Text Full Text (PDF) Supplemental Methods and Figures All Versions of this Article: blood-2007-12-128215v1 112/2/350    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Google Scholar Articles by Zeoli, A. Articles by Brizzi, M. F. PubMed PubMed Citation Articles by Zeoli, A. Articles by Brizzi, M. F. Related Collections Hemostasis, Thrombosis, and Vascular Biology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Interleukin-3 promotes expansion of hemopoietic-derived CD45+ angiogenic cells and their arterial commitment via STAT5 activation Annarita Zeoli1, Patrizia Dentelli1, Arturo Rosso1, Gabriele Togliatto1, Antonella Trombetta1, Laura Damiano2, Paola Francia di Celle3, Luigi Pegoraro1, Fiorella Altruda2, and Maria Felice Brizzi1 1 Department of Internal Medicine, University of Torino, Torino; 2 Department of Genetics, Biology and Biochemistry, Molecular Biotechnology Center, University of Torino, Torino; and 3 Center for Experimental Research and Clinical Studies (CERMS), Azienda Ospedaliera S. Giovanni Battista, Torino, Italy Interleukin-3 (IL-3) released by infiltrating inflammatory cells in different pathologic settings contributes to organ and tumor angiogenesis. Here we demonstrate that IL-3 expands a subset of CD45+ circulating angiogenic cells clonally derived from the hemopoietic progenitors. Moreover, CD45+ cells exposed to IL-3 acquire arterial specification and contribute to the formation of vessels in vivo. Depletion of signal transducer and activator of transcription 5 (STAT5) provides evidence that IL-3–mediated cell expansion and arterial morphogenesis rely on STAT5 activation. In addition, by means of Tie2-transgenic mice, we demonstrate that STAT5 also regulates IL-3–induced expansion and arterial specification of bonemarrow–derived CD45+ cells. Thus, our data provide the first evidence that, in inflammatory microenvironments containing IL-3, angiogenic cells derived from hemopoietic precursors can act as adult vasculogenic cells. Moreover, the characterization of the signaling pathway regulating these events provides the rationale for therapeutically targeting STAT5 in these pathologic settings. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

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