SEARCH:   Advanced

Blood, 15 July 2008, Vol. 112, No. 2, pp. 394-397. Prepublished online as a Blood First Edition Paper on April 23, 2008; DOI 10.1182/blood-2007-11-124065. This Article Full Text Full Text (PDF) Supplemental Tables and Figures All Versions of this Article: blood-2007-11-124065v1 112/2/394    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Proto-Siqueira, R. Articles by Gribben, J. G. Search for Related Content PubMed PubMed Citation Articles by Proto-Siqueira, R. Articles by Gribben, J. G. Related Collections Neoplasia Brief Reports Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  NEOPLASIA Brief Report SAGE analysis demonstrates increased expression of TOSO contributing to Fas-mediated resistance in CLL Rodrigo Proto-Siqueira1,2, Rodrigo A. Panepucci1, Francisco P. Careta1, Abigail Lee2, Andrew Clear2, Kelly Morris2, Carolyn Owen2, Edgar G. Rizzatti1, Wilson A. Silva, Jr1, Roberto P. Falcão1, Marco A. Zago1, and John G. Gribben2 1 Hematology Division and Center for Cell-Based Therapy, Faculty of Medicine of Ribeirão Preto, University of São Paulo, São Paulo, Brazil; and 2 Institute of Cancer, Barts and The London School of Medicine and Dentistry, London, United Kingdom To identify novel genes involved in the molecular pathogenesis of chronic lymphocytic leukemia (CLL) we performed a serial analysis of gene expression (SAGE) in CLL cells, and compared this with healthy B cells (nCD19+). We found a high level of similarity among CLL subtypes, but a comparison of CLL versus nCD19+ libraries revealed 55 genes that were over-represented and 49 genes that were down-regulated in CLL. A gene ontology analysis revealed that TOSO, which plays a functional role upstream of Fas extrinsic apoptosis pathway, was over-expressed in CLL cells. This finding was confirmed by real-time reverse transcription–polymerase chain reaction in 78 CLL and 12 nCD19+ cases (P < .001). We validated expression using flow cytometry and tissue microarray and demonstrated a 5.6-fold increase of TOSO protein in circulating CLL cells (P = .013) and lymph nodes (P = .006). Our SAGE results have demonstrated that TOSO is a novel over-expressed antiapoptotic gene in CLL. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: H. Kubagawa, S. Oka, Y. Kubagawa, I. Torii, E. Takayama, D.-W. Kang, G. L. Gartland, L. F. Bertoli, H. Mori, H. Takatsu, et al. Identity of the elusive IgM Fc receptor (Fc{micro}R) in humans J. Exp. Med., October 26, 2009; (2009) jem.20091107v1. [Abstract] [Full Text] [PDF] C. P. Pallasch, A. Schulz, N. Kutsch, J. Schwamb, S. Hagist, H. Kashkar, A. Ultsch, C. Wickenhauser, M. Hallek, and C.-M. Wendtner Overexpression of TOSO in CLL is triggered by B-cell receptor signaling and associated with progressive disease Blood, November 15, 2008; 112(10): 4213 - 4219. [Abstract] [Full Text] [PDF]

	Copyright © 2008 by American Society of Hematology         Online ISSN: 1528-0020