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 Blood, 15 August 2008, Vol. 112, No. 4, pp. 1048-1055.
Prepublished online as a Blood First Edition Paper on May 21, 2008; DOI 10.1182/blood-2007-11-125385.

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HEMATOPOIESIS AND STEM CELLS

B-lineage commitment prior to surface expression of B220 and CD19 on hematopoietic progenitor cells

Robert Mansson13, Sasan Zandi1, Kristina Anderson2,3, Inga-Lill Martensson4, Sten Eirik W. Jacobsen2,3, David Bryder5, and Mikael Sigvardsson13

1 Department for Biomedicine and Surgery, Linköping University, Linköping, Sweden; 2 Hematopoietic Stem Cell Laboratory and 3 Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University, Lund, Sweden; 4 Laboratory of Lymphocyte Signaling and Development, The Babraham Institute, Cambridge, United Kingdom; and 5 Department of Immunology, Lund University, Lund, Sweden

Commitment of hematopoietic progenitor cells to B-lymphoid cell fate has been suggested to coincide with the development of PAX5-expressing B220+CD19+ pro–B cells. We have used a transgenic reporter mouse, expressing human CD25 under the control of the B-lineage–restricted Igll1 ({lambda}5) promoter to investigate the lineage potential of early progenitor cells in the bone marrow. This strategy allowed us to identify a reporter expressing LIN–B220–CD19–CD127+FLT3+SCA1lowKITlow population that displays a lack of myeloid and a 90% reduction in in vitro T-cell potential compared with its reporter-negative counterpart. Gene expression analysis demonstrated that these lineage-restricted cells express B-lineage–associated genes to levels comparable with that observed in pro–B cells. These data suggest that B-lineage commitment can occur before the expression of B220 and CD19.


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