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Blood, 15 August 2008, Vol. 112, No. 4, pp. 1078-1084. Prepublished online as a Blood First Edition Paper on June 2, 2008; DOI 10.1182/blood-2008-02-139402. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2008-02-139402v1 112/4/1078    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Olsson, B. Articles by Wadenvik, H. Search for Related Content PubMed PubMed Citation Articles by Olsson, B. Articles by Wadenvik, H. Related Collections Hemostasis, Thrombosis, and Vascular Biology Immunobiology Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Recruitment of T cells into bone marrow of ITP patients possibly due to elevated expression of VLA-4 and CX3CR1 Bob Olsson1, Börje Ridell2, Lena Carlsson3, Stefan Jacobsson4, and Hans Wadenvik1 Departments of1 Internal Medicine, 2 Pathology, 3 Molecular and Clinical Medicine, and 4 Clinical Chemistry and Transfusion Medicine, the Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden In idiopathic thrombocytopenic purpura (ITP), platelets are destroyed in the spleen, liver, and bone marrow (BM) by autoantibodies and cytotoxic T cells. In a DNA microarray screen of peripheral blood T cells, we found that VLA-4, CX3CR1, and CXCR4, involved in T-cell homing, had increased expression in ITP patients compared with controls. However, we only found increased protein expression of VLA-4 on T cells from peripheral blood by flow cytometry. To address a possible recruitment of T cells into the organs involved in platelet destruction, we analyzed T cells in BM. In BM, T-cell surface expression of VLA-4 and CX3CR1 was increased in ITP patients compared with controls. Furthermore, the number of CD3+ T cells in BM, but not in blood, was increased in ITP patients compared with controls. This finding was confirmed by immunohistochemistry of BM biopsies. The number of regulatory T cells (CD4+/CD25bright) was decreased in the BM of ITP patients, whereas Fas expression was increased. In conclusion, ITP is associated with accumulation and activation of T cells in the BM. Recruitment of T cells into the target organ (eg, BM) is plausible and may be facilitated through increased VLA-4 and CX3CR1 expression. These molecules might serve as new treatment targets in ITP. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: ITP three R's: regulation, routing, rituximab John W. Semple Blood 2008 112: 927-928. [Full Text] [PDF] This article has been cited by other articles: D. B. Cines, J. B. Bussel, H. A. Liebman, and E. T. Luning Prak The ITP syndrome: pathogenic and clinical diversity Blood, June 25, 2009; 113(26): 6511 - 6521. [Abstract] [Full Text] [PDF] J. W. Semple Platelet antigen-induced regulation in ITP Blood, March 12, 2009; 113(11): 2373 - 2373. [Full Text] [PDF]

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