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Blood, 15 August 2008, Vol. 112, No. 4, pp. 1280-1289. Prepublished online as a Blood First Edition Paper on June 4, 2008; DOI 10.1182/blood-2008-01-134429. This Article Full Text Full Text (PDF) Supplemental Videos All Versions of this Article: blood-2008-01-134429v1 112/4/1280    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Stefanidakis, M. Articles by Luscinskas, F. W. Search for Related Content PubMed PubMed Citation Articles by Stefanidakis, M. Articles by Luscinskas, F. W. Related Collections Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Endothelial CD47 interaction with SIRP is required for human T-cell transendothelial migration under shear flow conditions in vitro Michael Stefanidakis1, Gail Newton1, Winston Y. Lee2, Charles A. Parkos2, and Francis W. Luscinskas1 1 Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA; and 2 Epithelial Pathobiology Research Unit, Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA Leukocyte transendothelial migration (TEM) is a critical event during inflammation. CD47 has been implicated in myeloid cell migration across endothelium and epithelium. CD47 binds to signal regulatory protein (SIRP), SIRP and SIRP. So far, little is known about the role of endothelial CD47 in T-cell TEM in vivo or under flow conditions in vitro. Fluorescence-activated cell sorting and biochemical analysis show that CD3+ T cells express SIRP but not SIRP, and fluorescence microscopy showed that CD47 was enriched at endothelial junctions. These expression patterns suggested that CD47 plays a role in T-cell TEM through binding interactions with SIRP. We tested, therefore, whether CD47-SIRP interactions affect T-cell transmigration using blocking mAb against CD47 or SIRP in an in vitro flow model. These antibodies inhibited T-cell TEM by 70% plus or minus 6% and 82% plus or minus 1%, respectively, but had no effect on adhesion. In agreement with human mAb studies, transmigration of murine wild-type T helper type 1 cells across TNF-–activated murine CD47–/– endothelium was reduced by 75% plus or minus 2% even though murine T cells appear to lack SIRP. Nonetheless, these findings suggest endothelial cell CD47 interacting with T-cell ligands, such as SIRP, play an important role in T-cell transendothelial migration. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: W. A. Muller Mechanisms of Transendothelial Migration of Leukocytes Circ. Res., July 31, 2009; 105(3): 223 - 230. [Abstract] [Full Text] [PDF]

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