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Blood, 1 January 2009, Vol. 113, No. 1, pp. 58-65. Prepublished online as a Blood First Edition Paper on October 10, 2008; DOI 10.1182/blood-2008-07-168393. This Article Full Text Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2008-07-168393v1 113/1/58    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bosque, A. Articles by Planelles, V. Search for Related Content PubMed PubMed Citation Articles by Bosque, A. Articles by Planelles, V. Related Collections Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Induction of HIV-1 latency and reactivation in primary memory CD4+ T cells Alberto Bosque1, and Vicente Planelles1 1 Department of Pathology, University of Utah, Salt Lake City The use of antiretroviral therapy in HIV type 1 (HIV-1)–infected patients does not lead to virus eradication. This is due, to a significant degree, to the fact that HIV-1 can establish a highly stable reservoir of latently infected cells. In this work, we describe an ex vivo experimental system that generates high levels of HIV-1 latently infected memory cells using primary CD4+ T cells. Using this model, we were able to dissect the T cell–signaling pathways and to characterize the long terminal repeat (LTR) cis-acting elements involved in reactivation of HIV-1 in memory CD4+ T cells. We conclude that Lck and nuclear factor of activated T cells (NFAT), but not NF-B, are required for optimal latent virus reactivation in memory T cells. We also found that the cis-acting elements which are critical toward HIV-1 reactivation are the Sp1 and B/NFAT transcription factor binding sites. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

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