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Blood, 12 March 2009, Vol. 113, No. 11, pp. 2568-2577. Prepublished online as a Blood First Edition Paper on December 3, 2008; DOI 10.1182/blood-2008-03-148288. This Article Full Text Full Text (PDF) Supplemental Tables and Figure All Versions of this Article: blood-2008-03-148288v1 113/11/2568    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Zhang, X.-L. Articles by Hou, M. Search for Related Content PubMed PubMed Citation Articles by Zhang, X.-L. Articles by Hou, M. Related Collections Immunobiology Platelets and Thrombopoiesis Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  PLATELETS AND THROMBOPOIESIS De novo induction of platelet-specific CD4+CD25+ regulatory T cells from CD4+CD25– cells in patients with idiopathic thrombocytopenic purpura Xiao-Lin Zhang1,*, Jun Peng1,2,*, Jian-Zhi Sun1, Jia-Jun Liu3, Cheng-Shan Guo4, Zhen-Guang Wang5, Yuan Yu1, Yan Shi1, Ping Qin1, Shu-Guang Li1, Li-Ning Zhang6, and Ming Hou1,2 1 Department of Hematology, Qilu Hospital, Shandong University, Jinan; 2 Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health, Jinan; 3 Department of Hematology, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou; 4 Department of Hematology, Second Hospital, Shandong University, Jinan; 5 Department of Science and Technology, Shandong University, Jinan; and 6 Institute of Immunology, School of Medicine, Shandong University, Jinan, Shandong, People's Republic of China CD4+CD25+ regulatory T cells (Treg) play the critical role in maintenance of peripheral immune tolerance. However, the numbers of naturally occurring Treg (nTreg) that can be isolated from periphery are far too small to be clinically effective. The isolation and expansion of nTreg for treatment of autoimmune diseases encounter great difficulties. Whether autoantigen-specific Treg could be converted from CD4+CD25– T cells in patients with autoimmune diseases has not been reported. Here, we demonstrated that platelet glycoprotein (GP)–specific induced Treg (GP-iTreg) could be generated de novo from nonregulatory CD4+CD25–CD45RA+ cells in patients with idiopathic thrombocytopenic purpura and induced both antigen-specific and linked suppression. GP-iTreg mediated regulatory effects via modulating the T cell–stimulatory capacity of dendritic cells. By investigating the gene expression profile of iTreg-modulated dendritic cells, we provided a genome-wide assessment of the changes induced by antigen-specific iTreg and identified that the Toll-like receptor, Notch and transforming growth factor-β signaling pathways were related to the GP-specific tolerance, with the Toll-like receptor pathway being dominant. The findings in patients with idiopathic thrombocytopenic purpura will facilitate our understanding of the mechanisms of induction and maintenance of autoantigen-specific tolerance and highlight the considerable potential of antigen-specific iTreg for targeted immunotherapy in human auto-immune diseases. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Platelet antigen-induced regulation in ITP John W. Semple Blood 2009 113: 2373. [Full Text] [PDF] This article has been cited by other articles: J. W. Semple Platelet antigen-induced regulation in ITP Blood, March 12, 2009; 113(11): 2373 - 2373. [Full Text] [PDF]

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