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Blood, 26 March 2009, Vol. 113, No. 13, pp. 2914-2923. Prepublished online as a Blood First Edition Paper on December 18, 2008; DOI 10.1182/blood-2008-07-167106. This Article Full Text Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2008-07-167106v1 113/13/2914    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Yokota, T. Articles by Kanakura, Y. Search for Related Content PubMed PubMed Citation Articles by Yokota, T. Articles by Kanakura, Y. Related Collections Hematopoiesis and Stem Cells Vascular Biology Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS AND STEM CELLS The endothelial antigen ESAM marks primitive hematopoietic progenitors throughout life in mice Takafumi Yokota1, Kenji Oritani1, Stefan Butz2, Koichi Kokame3, Paul W. Kincade4, Toshiyuki Miyata3, Dietmar Vestweber2, and Yuzuru Kanakura1 1 Department of Hematology and Oncology, Osaka University Graduate School of Medicine, Suita, Japan; 2 Department of Vascular Cell Biology, Max-Planck-Institute for Molecular Biomedicine, Münster, Germany; 3 National Cardiovascular Center Research Institute, Osaka, Japan; and 4 Immunobiology and Cancer Program, Oklahoma Medical Research Foundation, Oklahoma City Although recent advances have enabled hematopoietic stem cells (HSCs) to be enriched to near purity, more information about their characteristics will improve our understanding of their development and stage-related functions. Here, using microarray technology, we identified endothelial cell-selective adhesion molecule (ESAM) as a novel marker for murine HSCs in fetal liver. Esam was expressed at high levels within a Rag1– c-kitHi Sca1+ HSC-enriched fraction, but sharply down-regulated with activation of the Rag1 locus, a valid marker for the most primitive lymphoid progenitors in E14.5 liver. The HSC-enriched fraction could be subdivided into 2 on the basis of ESAM levels. Among endothelial antigens on hematopoietic progenitors, ESAM expression showed intimate correlation with HSC activity. The ESAMHi population was highly enriched for multipotent myeloid-erythroid progenitors and primitive progenitors with lymphopoietic activity, and exclusively reconstituted long-term lymphohematopoiesis in lethally irradiated recipients. Tie2+ c-kit+ lymphohematopoietic cells in the E9.5–10.5 aorta-gonad-mesonephros region also expressed high levels of ESAM. Furthermore, ESAM was detected on primitive hematopoietic progenitors in adult bone marrow. Interestingly, ESAM expression in the HSC-enriched fraction was up-regulated in aged mice. We conclude that ESAM marks HSC in murine fetal liver and will facilitate studies of hematopoiesis throughout life. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: ESAM: adding to the hematopoietic toolbox Hanna Mikkola Blood 2009 113: 2871-2872. [Full Text] [PDF] This article has been cited by other articles: H. Mikkola ESAM: adding to the hematopoietic toolbox Blood, March 26, 2009; 113(13): 2871 - 2872. [Full Text] [PDF]

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