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 Blood, 2 April 2009, Vol. 113, No. 14, pp. 3323-3332.
Prepublished online as a Blood First Edition Paper on January 28, 2009; DOI 10.1182/blood-2008-06-162248.

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MYELOID NEOPLASIA

Runx2 induces acute myeloid leukemia in cooperation with Cbfβ-SMMHC in mice

Ya-Huei Kuo1, Sayyed K. Zaidi2, Svetlana Gornostaeva1, Toshihisa Komori3, Gary S. Stein2, and Lucio H. Castilla1

1 Program in Gene Function and Expression and 2 Department of Cell Biology, University of Massachusetts Medical School, Worcester; and 3 Department of Cell Biology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan

The core-binding factor (CBF) is a master regulator of developmental and differentiation programs, and CBF alterations are frequently associated with acute leukemia. The role of the CBF member RUNX2 in hematopoiesis is poorly understood. Genetic evidence suggests that deregulation of Runx2 may cause myeloid leukemia in mice expressing the fusion oncogene Cbfb-MYH11. In this study, we show that sustained expression of Runx2 modulates Cbfβ-smooth muscle myosin heavy chain (SMMHC)–mediated myeloid leukemia development. Expression of Runx2 is high in the hematopoietic stem cell compartment and decreases during myeloid differentiation. Sustained Runx2 expression hinders myeloid progenitor differentiation capacity and represses expression of CBF targets Csf1R, Mpo, Cebpd, the cell cycle inhibitor Cdkn1a, and myeloid markers Cebpa and Gfi1. In addition, full-length Runx2 cooperates with Cbfβ-SMMHC in leukemia development in transplantation assays. Furthermore, we show that the nuclear matrix–targeting signal and DNA-binding runt-homology domain of Runx2 are essential for its leukemogenic activity. Conversely, Runx2 haplo-insufficiency delays the onset and reduces the incidence of acute myeloid leukemia. Together, these results indicate that Runx2 is expressed in the stem cell compartment, interferes with differentiation and represses CBF targets in the myeloid compartment, and modulates the leukemogenic function of Cbfβ-SMMHC in mouse leukemia.


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