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Blood, 16 April 2009, Vol. 113, No. 16, pp. 3716-3725. Prepublished online as a Blood First Edition Paper on November 18, 2008; DOI 10.1182/blood-2008-09-179754. This Article Full Text Full Text (PDF) Supplemental Table and Figures All Versions of this Article: blood-2008-09-179754v1 113/16/3716    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bruhns, P. Articles by Daëron, M. Search for Related Content PubMed PubMed Citation Articles by Bruhns, P. Articles by Daëron, M. Related Collections Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Specificity and affinity of human Fc receptors and their polymorphic variants for human IgG subclasses Pierre Bruhns1,2, Bruno Iannascoli1,2, Patrick England3,4, David A. Mancardi1,2, Nadine Fernandez5, Sylvie Jorieux6, and Marc Daëron1,2 1 Institut Pasteur, Département d'Immunologie, Unité d'Allergologie Moléculaire et Cellulaire, Paris; 2 Inserm, U760, Paris; 3 Institut Pasteur, Département de Biologie Structurale et Chimie, Plateforme de Biophysique des Macromolécules et de leurs Interactions, Paris; 4 Centre National de la Recherche Scientifique (CNRS), URA 2185, Paris; 5 Laboratoire Français du fractionnement et des Biotechnologies, Les Ulis; and 6 Laboratoire Français du fractionnement et des Biotechnologies, Lille, France Distinct genes encode 6 human receptors for IgG (hFcRs), 3 of which have 2 or 3 polymorphic variants. The specificity and affinity of individual hFcRs for the 4 human IgG subclasses is unknown. This information is critical for antibody-based immunotherapy which has been increasingly used in the clinics. We investigated the binding of polyclonal and monoclonal IgG1, IgG2, IgG3, and IgG4 to FcRI; FcRIIA, IIB, and IIC; FcRIIIA and IIIB; and all known polymorphic variants. Wild-type and low-fucosylated IgG1 anti-CD20 and anti-RhD mAbs were also examined. We found that (1) IgG1 and IgG3 bind to all hFcRs; (2) IgG2 bind not only to FcRIIAH131, but also, with a lower affinity, to FcRIIAR131 and FcRIIIAV158; (3) IgG4 bind to FcRI, FcRIIA, IIB and IIC and FcRIIIAV158; and (4) the inhibitory receptor FcRIIB has a lower affinity for IgG1, IgG2, and IgG3 than all other hFcRs. We also identified parameters that determine the specificity and affinity of hFcRs for IgG subclasses. These results document how hFcR specificity and affinity may account for the biological activities of antibodies. They therefore highlight the role of specific hFcRs in the therapeutic and pathogenic effects of antibodies in disease. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. A. Ingersoll, R. Spanbroek, C. Lottaz, E. L. Gautier, M. Frankenberger, R. Hoffmann, R. Lang, M. Haniffa, M. Collin, F. Tacke, et al. Comparison of gene expression profiles between human and mouse monocyte subsets Blood, January 21, 2010; 115(3): e10 - e19. [Abstract] [Full Text] [PDF] C. A. Saylor, E. Dadachova, and A. Casadevall Murine IgG1 and IgG3 Isotype Switch Variants Promote Phagocytosis of Cryptococcus neoformans through Different Receptors J. Immunol., January 1, 2010; 184(1): 336 - 343. [Abstract] [Full Text] [PDF] T. Schneider-Merck, J. J. Lammerts van Bueren, S. Berger, K. Rossen, P. H.C. van Berkel, S. Derer, T. Beyer, S. Lohse, W. K. Bleeker, M. Peipp, et al. 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Hudrisier, B. Clemenceau, S. Balor, S. Daubeuf, E. Magdeleine, M. Daeron, P. Bruhns, and H. Vie Ligand Binding but Undetected Functional Response of FcR after Their Capture by T Cells via Trogocytosis J. Immunol., November 15, 2009; 183(10): 6102 - 6113. [Abstract] [Full Text] [PDF] L. G. Perez, M. R. Costa, C. A. Todd, B. F. Haynes, and D. C. Montefiori Utilization of Immunoglobulin G Fc Receptors by Human Immunodeficiency Virus Type 1: a Specific Role for Antibodies against the Membrane-Proximal External Region of gp41 J. Virol., August 1, 2009; 83(15): 7397 - 7410. [Abstract] [Full Text] [PDF]

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