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Blood, 16 April 2009, Vol. 113, No. 16, pp. 3838-3844. Prepublished online as a Blood First Edition Paper on January 9, 2009; DOI 10.1182/blood-2008-09-178475. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2008-09-178475v1 113/16/3838    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ahlen, M. T. Articles by Stuge, T. B. Search for Related Content PubMed PubMed Citation Articles by Ahlen, M. T. Articles by Stuge, T. B. Related Collections Immunobiology Platelets and Thrombopoiesis Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  PLATELETS AND THROMBOPOIESIS T-cell responses associated with neonatal alloimmune thrombocytopenia: isolation of HPA-1a–specific, HLA-DRB3*0101–restricted CD4+ T cells Maria Therese Ahlen1,2, Anne Husebekk1,2, Mette Kjær Killie1, Bjørn Skogen1,2, and Tor B. Stuge1,2 1 Department of Immunology and Transfusion Medicine, University Hospital of North Norway, Tromsø; and 2 Department of Immunology, University of Tromsø, Tromsø, Norway T-cell responses have been implicated in the development of HPA-1a–induced neonatal alloimmune thrombocytopenia (NAIT). However, HPA-1a–specific T cells have neither been isolated nor characterized. Here, we aimed to determine whether HPA-1a–specific T cells could be isolated from HPA-1a–immunized women. In the present study, peripheral blood mononuclear cells (PBMCs) from an HPA-1a–alloimmunized woman were cultured for weeks in the presence of HPA-1a peptide, labeled with CFSE, and assayed for antigen-specific proliferation. Individual proliferating cells were isolated by fluorescence-activated cell sorting and expanded in culture. Antigen specificity and HLA restriction were determined by cytokine secretion (enzyme-linked immunospot [ELISPOT]) and proliferation assays. Several CD3+CD4+ T-cell clones were isolated that proliferated and secreted cytokines in response to HPA-1a peptide. Two of these clones have been established in long-term culture in our laboratory. Both of these recognize synthetic as well as naturally processed HPA-1a antigen, and the recognition is restricted by the MHC molecule HLA-DRB3*0101 that is strongly associated with NAIT. These HPA-1a–specific T-cell clones represent unambiguous evidence for the association of T-cell responses with NAIT, and they will serve as unique tools to elucidate the cellular immune response that may result in NAIT. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. W. Semple Processed platelet HPA1a peptides au naturel Blood, August 27, 2009; 114(9): 1724 - 1725. [Abstract] [Full Text] [PDF]

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