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 Blood, 23 April 2009, Vol. 113, No. 17, pp. 3953-3960.
Prepublished online as a Blood First Edition Paper on February 12, 2009; DOI 10.1182/blood-2008-10-182105.

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HEMATOPOIESIS AND STEM CELLS

Human and murine amniotic fluid c-Kit+Lin– cells display hematopoietic activity

Andrea Ditadi13, Paolo de Coppi3,4, Olivier Picone57, Laetitia Gautreau2,8, Rim Smati2,8, Emmanuelle Six1,2, Delphine Bonhomme1,2, Sophie Ezine2,8, René Frydman57, Marina Cavazzana-Calvo1,2,9,*, and Isabelle André-Schmutz1,2,*

1 Inserm U768, Paris, France; 2 Faculty of Medicine, University of Paris René Descartes, IFR94, Paris, France; 3 Stem Cell Processing Laboratory, Department of Pediatrics, University of Padua, Padua, Italy; 4 Surgery Unit, UCL Institute of Child Health and Great Ormond Street Hospital, London, United Kingdom; 5 Assistance Publique-Hopitaux de Paris, Gynecology & Obstetrics Department, Antoine Béclère Hospital, Clamart, France; 6 University of Paris-Sud, Clamart, France; 7 Inserm U782, Clamart, France; 8 Inserm U591, Paris, France; and 9 Biotherapy Department, Assistance Publique-Hopitaux de Paris, Necker Children's Hospital, Paris, France

We have isolated c-Kit+Lin– cells from both human and murine amniotic fluid (AF) and investigated their hematopoietic potential. In vitro, the c-Kit+Lin– population in both species displayed a multilineage hematopoietic potential, as demonstrated by the generation of erythroid, myeloid, and lymphoid cells. In vivo, cells belonging to all 3 hematopoietic lineages were found after primary and secondary transplantation of murine c-Kit+Lin– cells into immunocompromised hosts, thus demonstrating the ability of these cells to self-renew. Gene expression analysis of c-Kit+ cells isolated from murine AF confirmed these results. The presence of cells with similar characteristics in the surrounding amnion indicates the possible origin of AF c-Kit+Lin– cells. This is the first report showing that cells isolated from the AF do have hematopoietic potential; our results support the idea that AF may be a new source of stem cells for therapeutic applications.


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