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Blood, 23 April 2009, Vol. 113, No. 17, pp. 4011-4015. Prepublished online as a Blood First Edition Paper on January 14, 2009; DOI 10.1182/blood-2008-10-183483. This Article Full Text Full Text (PDF) Supplemental Materials and Methods All Versions of this Article: blood-2008-10-183483v1 113/17/4011    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Burmeister, T. Articles by Marschalek, R. Search for Related Content PubMed PubMed Citation Articles by Burmeister, T. Articles by Marschalek, R. Related Collections Lymphoid Neoplasia Clinical Trials and Observations Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  LYMPHOID NEOPLASIA The MLL recombinome of adult CD10-negative B-cell precursor acute lymphoblastic leukemia: results from the GMALL study group Thomas Burmeister1, Claus Meyer2, Stefan Schwartz1, Julia Hofmann2, Mara Molkentin1, Eric Kowarz2, Björn Schneider3, Thorsten Raff4, Richard Reinhardt5, Nicola Gökbuget6, Dieter Hoelzer6, Eckhard Thiel1, and Rolf Marschalek2 1 Charité Universitätsmedizin Berlin, Campus Benjamin Franklin (CBF), Medizinische Klinik III, Berlin; 2 Institut für Pharmazeutische Biologie/ZAFES/Diagnostikzentrum für Akute Leukämie (DCAL), Goethe-Universität, Frankfurt/Main; 3 Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ), Braunschweig; 4 Medizinische Klinik II, Universitätsklinikum Schleswig-Holstein (UKSH), Campus Kiel, Kiel; 5 Max Planck-Institut (MPI) für Molekulare Genetik, Berlin; and 6 Goethe-Universität, Medizinische Klinik II, Frankfurt, Germany MLL translocations in adult B-cell precursor (BCP) acute lymphoblastic leukemia (ALL) are largely restricted to the immature CD10– immunophenotypes. MLL-AF4 is known to be the most frequent fusion transcript, but the exact frequencies of MLL aberrations in CD10– adult BCP-ALL are unknown. We present a genetic characterization of 184 BCR-ABL– CD10– adult ALL cases (156 cyIg–, 28 cyIg+) diagnosed between 2001 and 2007 at the central diagnostic laboratory of the GMALL study group. Patient samples were investigated by RT-PCR for MLL-AF4, MLL-ENL, and MLL-AF9 and by long-distance inverse polymerase chain reaction, thus also allowing the identification of unknown MLL fusion partners at the genomic level. MLL-AF4 was detected in 101 (54.9%) and MLL-ENL in 11 (6.0%) cases. In addition, rare MLL fusion genes were found: 2 MLL-TET1 cases, not previously reported in ALL, 1 MLL-AF9, 1 MLL-PTD, a novel MLL-ACTN4, and an MLL-11q23 fusion. Chromosomal breakpoints were determined in all 118 positive cases, revealing 2 major breakpoint cluster regions in the MLL gene. Characteristic features of MLL+ patients were significantly lower CD10 expression, expression of the NG2 antigen, a higher white blood count at diagnosis, and female sex. Proposals are made for diagnostic assessment. The clinical studies are registered at http://www.clinicaltrials.gov as NCT00199056 [ClinicalTrials.gov] and NCT00198991 [ClinicalTrials.gov] . CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: R. K. Slany The molecular biology of mixed lineage leukemia Haematologica, July 1, 2009; 94(7): 984 - 993. [Abstract] [Full Text] [PDF]

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