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Blood, 7 May 2009, Vol. 113, No. 19, pp. 4586-4594. Prepublished online as a Blood First Edition Paper on February 6, 2009; DOI 10.1182/blood-2008-09-178186. This Article Full Text Full Text (PDF) Supplemental Methods, Tables, and Figures All Versions of this Article: blood-2008-09-178186v1 113/19/4586    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Zhang, J. Articles by Dave, S. S. Search for Related Content PubMed PubMed Citation Articles by Zhang, J. Articles by Dave, S. S. Related Collections Immunobiology Lymphoid Neoplasia Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Patterns of microRNA expression characterize stages of human B-cell differentiation Jenny Zhang1,*, Dereje D. Jima1,*, Cassandra Jacobs1, Randy Fischer2, Eva Gottwein3, Grace Huang1, Patricia L. Lugar2,4, Anand S. Lagoo5, David A. Rizzieri4, Daphne R. Friedman4, J. Brice Weinberg4, Peter E. Lipsky2, and Sandeep S. Dave1,4 1 Duke Institute for Genome Sciences and Policy, Duke University, Durham, NC; 2 Autoimmunity Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, Bethesda, MD; and Departments of 3 Molecular Genetics and Microbiology and Center for Virology, 4 Medicine, and 5 Pathology, Duke University Medical Center, Durham, NC Mature B-cell differentiation provides an important mechanism for the acquisition of adaptive immunity. Malignancies derived from mature B cells constitute the majority of leukemias and lymphomas. These malignancies often maintain the characteristics of the normal B cells that they are derived from, a feature that is frequently used in their diagnosis. The role of microRNAs in mature B cells is largely unknown. Through concomitant microRNA and mRNA profiling, we demonstrate a potential regulatory role for microRNAs at every stage of the mature B-cell differentiation process. In addition, we have experimentally identified a direct role for the microRNA regulation of key transcription factors in B-cell differentiation: LMO2 and PRDM1 (Blimp1). We also profiled the microRNA of B-cell tumors derived from diffuse large B-cell lymphoma, Burkitt lymphoma, and chronic lymphocytic leukemia. We found that, in contrast to many other malignancies, common B-cell malignancies do not down-regulate microRNA expression. Although these tumors could be distinguished from each other with use of microRNA expression, each tumor type maintained the expression of the lineage-specific microRNAs. Expression of these lineage-specific microRNAs could correctly predict the lineage of B-cell malignancies in more than 95% of the cases. Thus, our data demonstrate that microRNAs may be important in maintaining the mature B-cell phenotype in normal and malignant B cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

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