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 Blood, 21 May 2009, Vol. 113, No. 21, pp. 5125-5133.
Prepublished online as a Blood First Edition Paper on March 18, 2009; DOI 10.1182/blood-2009-01-199950.

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IMMUNOBIOLOGY

Selective expression of latency-associated peptide (LAP) and IL-1 receptor type I/II (CD121a/CD121b) on activated human FOXP3+ regulatory T cells allows for their purification from expansion cultures

Dat Q. Tran1, John Andersson1, Donna Hardwick2, Lolita Bebris2, Gabor G. Illei2, and Ethan M. Shevach1

1 Laboratory of Immunology, National Institute of Allergy and Infectious Diseases (NIAID) and 2 Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health (NIH), Bethesda, MD

Although adoptive transfer of regulatory T cells (Foxp3+ Tregs) has proven to be efficacious in the prevention and treatment of autoimmune diseases and graft-versus-host disease in rodents, a major obstacle for the use of Treg immunotherapy in humans is the difficulty of obtaining a highly purified preparation after ex vivo expansion. We have identified latency-associated peptide (LAP) and IL-1 receptor type I and II (CD121a/CD121b) as unique cell-surface markers that distinguish activated Tregs from activated FOXP3– and FOXP3+ non-Tregs. We show that it is feasible to sort expanded FOXP3+ Tregs from non-Tregs with the use of techniques for magnetic bead cell separation based on expression of these 3 markers. After separation, the final product contains greater than 90% fully functional FOXP3+ Tregs. This novel protocol should facilitate the purification of Tregs for both cell-based therapies as well as detailed studies of human Treg function in health and disease.


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This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
D. Q. Tran, J. Andersson, R. Wang, H. Ramsey, D. Unutmaz, and E. M. Shevach
GARP (LRRC32) is essential for the surface expression of latent TGF-{beta} on platelets and activated FOXP3+ regulatory T cells
PNAS, August 11, 2009; 106(32): 13445 - 13450.
[Abstract] [Full Text] [PDF]


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