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Blood, 28 May 2009, Vol. 113, No. 22, pp. 5650-5659. Prepublished online as a Blood First Edition Paper on April 3, 2009; DOI 10.1182/blood-2008-09-176776. This Article Full Text Full Text (PDF) Supplemental Tables and Figures Additional Supplemental Figures All Versions of this Article: blood-2008-09-176776v1 113/22/5650    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Google Scholar Articles by Kataru, R. P. Articles by Koh, G. Y. PubMed PubMed Citation Articles by Kataru, R. P. Articles by Koh, G. Y. Related Collections Immunobiology Phagocytes, Granulocytes, and Myelopoiesis Vascular Biology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  VASCULAR BIOLOGY Critical role of CD11b+ macrophages and VEGF in inflammatory lymphangiogenesis, antigen clearance, and inflammation resolution Raghu P. Kataru1, Keehoon Jung1, Cholsoon Jang1, Hanseul Yang1, Reto A. Schwendener2, Jung Eun Baik3, Seung Hyun Han3,4, Kari Alitalo5, and Gou Young Koh1 1 National Research Laboratory of Vascular Biology and Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Korea; 2 Laboratory of Liposome Research, Institute of Molecular Cancer Research, University of Zurich, Zurich, Switzerland; 3 Department of Oral Microbiology and Immunology, Dental Research Institute, School and BK21 Program of Dentistry, Seoul National University, Seoul, Korea; 4 International Vaccine Institute, Seoul, Korea; and 5 The Molecular/Cancer Biology Laboratory, Biomedicum Helsinki, University of Helsinki, Helsinki, Finland Using a bacterial pathogen–induced acute inflammation model in the skin, we defined the roles of local lymphatic vessels and draining lymph nodes (DLNs) in antigen clearance and inflammation resolution. At the peak day of inflammation, robust expansion of lymphatic vessels and profound infiltration of CD11b+/Gr-1+ macrophages into the inflamed skin and DLN were observed. Moreover, lymph flow and inflammatory cell migration from the inflamed skin to DLNs were enhanced. Concomitantly, the expression of lymphangiogenic growth factors such as vascular endothelial growth factor C (VEGF-C), VEGF-D, and VEGF-A were significantly up-regulated in the inflamed skin, DLNs, and particularly in enriched CD11b+ macrophages from the DLNs. Depletion of macrophages, or blockade of VEGF-C/D or VEGF-A, largely attenuated these phenomena, and produced notably delayed antigen clearance and inflammation resolution. Conversely, keratin 14 (K14)–VEGF-C transgenic mice, which have dense and enlarged lymphatic vessels in the skin dermis, exhibited accelerated migration of inflammatory cells from the inflamed skin to the DLNs and faster antigen clearance and inflammation resolution. Taken together, these results indicate that VEGF-C, -D, and -A derived from the CD11b+/Gr-1+ macrophages and local inflamed tissues play a critical role in promoting antigen clearance and inflammation resolution. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: K. E. Kim, Y.-J. Koh, B.-H. Jeon, C. Jang, J. Han, R. P. Kataru, R. A. Schwendener, J.-M. Kim, and G. Y. Koh Role of CD11b+ Macrophages in Intraperitoneal Lipopolysaccharide-Induced Aberrant Lymphangiogenesis and Lymphatic Function in the Diaphragm Am. J. Pathol., October 1, 2009; 175(4): 1733 - 1745. [Abstract] [Full Text] [PDF]

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