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Blood, 11 June 2009, Vol. 113, No. 24, pp. 6237-6245. Prepublished online as a Blood First Edition Paper on April 15, 2009; DOI 10.1182/blood-2009-02-205450. This Article Full Text Full Text (PDF) Supplemental Materials, Methods, and Figures All Versions of this Article: blood-2009-02-205450v1 113/24/6237    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Google Scholar Articles by Kodippili, G. C. Articles by Low, P. S. PubMed PubMed Citation Articles by Kodippili, G. C. Articles by Low, P. S. Related Collections Red Cells, Iron, and Erythropoiesis Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  RED CELLS, IRON, AND ERYTHROPOIESIS Imaging of the diffusion of single band 3 molecules on normal and mutant erythrocytes Gayani C. Kodippili1, Jeff Spector2, Caitlin Sullivan2,3, Frans A. Kuypers4, Richard Labotka5, Patrick G. Gallagher6, Ken Ritchie2, and Philip S. Low1 Departments of 1 Chemistry and 2 Physics, Purdue University, West Lafayette, IN; 3 Department of Physics, University of Illinois at Urbana-Champaign; 4 Children's Hospital Oakland, Research Institute, CA; 5 Division of Pediatric Hematology/Oncology, University of Illinois, Chicago; and 6 Department of Pediatrics, Yale University School of Medicine, New Haven, CT Membrane-spanning proteins may interact with a variety of other integral and peripheral membrane proteins via a diversity of protein-protein interactions. Not surprisingly, defects or mutations in any one of these interacting components can impact the physical and biological properties on the entire complex. Here we use quantum dots to image the diffusion of individual band 3 molecules in the plasma membranes of intact human erythrocytes from healthy volunteers and patients with defects in one of their membrane components, leading to well-known red cell pathologies (hereditary spherocytosis, hereditary elliptocytosis, hereditary hydrocytosis, Southeast Asian ovalocytosis, and hereditary pyropoikilocytosis). After characterizing the motile properties of the major subpopulations of band 3 in intact normal erythrocytes, we demonstrate that the properties of these subpopulations of band 3 change significantly in diseased cells, as evidenced by changes in the microscopic and macroscopic diffusion coefficients of band 3 and in the compartment sizes in which the different band 3 populations can diffuse. Because the above membrane abnormalities largely arise from defects in other membrane components (eg, spectrin, ankyrin), these data suggest that single particle tracking of band 3 might constitute a useful tool for characterizing the general structural integrity of the human erythrocyte membrane. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

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